Customized patterned substrates for highly versatile correlative light-scanning electron microscopy

Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical...

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Detalles Bibliográficos
Autores principales: Benedetti, Lorena, Sogne, Elisa, Rodighiero, Simona, Marchesi, Davide, Milani, Paolo, Francolini, Maura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229662/
https://www.ncbi.nlm.nih.gov/pubmed/25391455
http://dx.doi.org/10.1038/srep07033
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author Benedetti, Lorena
Sogne, Elisa
Rodighiero, Simona
Marchesi, Davide
Milani, Paolo
Francolini, Maura
author_facet Benedetti, Lorena
Sogne, Elisa
Rodighiero, Simona
Marchesi, Davide
Milani, Paolo
Francolini, Maura
author_sort Benedetti, Lorena
collection PubMed
description Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.
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spelling pubmed-42296622014-11-17 Customized patterned substrates for highly versatile correlative light-scanning electron microscopy Benedetti, Lorena Sogne, Elisa Rodighiero, Simona Marchesi, Davide Milani, Paolo Francolini, Maura Sci Rep Article Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy. Nature Publishing Group 2014-11-13 /pmc/articles/PMC4229662/ /pubmed/25391455 http://dx.doi.org/10.1038/srep07033 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Benedetti, Lorena
Sogne, Elisa
Rodighiero, Simona
Marchesi, Davide
Milani, Paolo
Francolini, Maura
Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
title Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
title_full Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
title_fullStr Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
title_full_unstemmed Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
title_short Customized patterned substrates for highly versatile correlative light-scanning electron microscopy
title_sort customized patterned substrates for highly versatile correlative light-scanning electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229662/
https://www.ncbi.nlm.nih.gov/pubmed/25391455
http://dx.doi.org/10.1038/srep07033
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