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Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway

BACKGROUND: Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/ma...

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Autores principales: Yeh, Tzu-Shao, Hsu, Cheng-Chen, Yang, Suh-Ching, Hsu, Mei-Chich, Liu, Jen-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229743/
https://www.ncbi.nlm.nih.gov/pubmed/24884709
http://dx.doi.org/10.1186/1472-6882-14-144
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author Yeh, Tzu-Shao
Hsu, Cheng-Chen
Yang, Suh-Ching
Hsu, Mei-Chich
Liu, Jen-Fang
author_facet Yeh, Tzu-Shao
Hsu, Cheng-Chen
Yang, Suh-Ching
Hsu, Mei-Chich
Liu, Jen-Fang
author_sort Yeh, Tzu-Shao
collection PubMed
description BACKGROUND: Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. METHODS: An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used. RESULT: The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P < 0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy. CONCLUSION: The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway.
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spelling pubmed-42297432014-11-14 Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway Yeh, Tzu-Shao Hsu, Cheng-Chen Yang, Suh-Ching Hsu, Mei-Chich Liu, Jen-Fang BMC Complement Altern Med Research Article BACKGROUND: Angelica Sinensis (AS), a folk medicine, has long been used in ergogenic aids for athletes, but there is little scientific evidence supporting its effects. We investigated whether AS induces hypertrophy in myotubes through the phosphatidylinositol 3-kinase (PI3K)/Akt (also termed PKB)/mammalian target of the rapamycin (mTOR) pathway. METHODS: An in vitro experiment investigating the induction of hypertrophy in myotubes was conducted. To investigate whether AS promoted the hypertrophy of myotubes, an established in vitro model of myotube hypertrophy with and without AS was used and examined using microscopic images. The role of the PI3K/Akt/mTOR signaling pathway in AS-induced myotube hypertrophy was evaluated. Two inhibitors, wortmannin (an inhibitor of PI3K) and rapamycin (an inhibitor of mTOR), were used. RESULT: The results revealed that the myotube diameters in the AS-treated group were significantly larger than those in the untreated control group (P < 0.05). Wortmannin and rapamycin inhibited AS-induced hypertrophy. Furthermore, AS increased Akt and mTOR phosphorylation through the PI3K pathway and induced myotube hypertrophy. CONCLUSION: The results confirmed that AS induces hypertrophy in myotubes through the PI3K/Akt/mTOR pathway. BioMed Central 2014-05-03 /pmc/articles/PMC4229743/ /pubmed/24884709 http://dx.doi.org/10.1186/1472-6882-14-144 Text en Copyright © 2014 Yeh et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yeh, Tzu-Shao
Hsu, Cheng-Chen
Yang, Suh-Ching
Hsu, Mei-Chich
Liu, Jen-Fang
Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway
title Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway
title_full Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway
title_fullStr Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway
title_full_unstemmed Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway
title_short Angelica Sinensis promotes myotube hypertrophy through the PI3K/Akt/mTOR pathway
title_sort angelica sinensis promotes myotube hypertrophy through the pi3k/akt/mtor pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229743/
https://www.ncbi.nlm.nih.gov/pubmed/24884709
http://dx.doi.org/10.1186/1472-6882-14-144
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