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Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa
BACKGROUND: Sperm DNA integrity is considered an important parameter to assess seminal fluid quality and can be used as a predictive test of potential fertility. Amongst the various tests to determine sperm DNA integrity, one is the Acridine Orange test. Recent studies have demonstrated the importan...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229798/ https://www.ncbi.nlm.nih.gov/pubmed/25395748 http://dx.doi.org/10.4103/0974-1208.142490 |
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author | Raimondo, Salvatore Gentile, Tommaso Cuomo, Felice De Filippo, Stefania Aprea, Gilda E. Guida, John |
author_facet | Raimondo, Salvatore Gentile, Tommaso Cuomo, Felice De Filippo, Stefania Aprea, Gilda E. Guida, John |
author_sort | Raimondo, Salvatore |
collection | PubMed |
description | BACKGROUND: Sperm DNA integrity is considered an important parameter to assess seminal fluid quality and can be used as a predictive test of potential fertility. Amongst the various tests to determine sperm DNA integrity, one is the Acridine Orange test. Recent studies have demonstrated the importance of p53 in maintaining sperm DNA integrity. The aim of this study was to assess if a p53 ELISA assay could be a new indicator of DNA damage in human spermatozoa. MATERIALS AND METHODS: 103 human semen samples were evaluated using both Acridine Orange test and p53 ELISA and results were compared. RESULTS: A clear correlation between the values measured by two methods was obtained. CONCLUSIONS: If this hypothesis will be confirmed by further studies, the p53 ELISA assay could become a new and more precise indicator of DNA damage in human spermatozoa. |
format | Online Article Text |
id | pubmed-4229798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42297982014-11-13 Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa Raimondo, Salvatore Gentile, Tommaso Cuomo, Felice De Filippo, Stefania Aprea, Gilda E. Guida, John J Hum Reprod Sci Original Article BACKGROUND: Sperm DNA integrity is considered an important parameter to assess seminal fluid quality and can be used as a predictive test of potential fertility. Amongst the various tests to determine sperm DNA integrity, one is the Acridine Orange test. Recent studies have demonstrated the importance of p53 in maintaining sperm DNA integrity. The aim of this study was to assess if a p53 ELISA assay could be a new indicator of DNA damage in human spermatozoa. MATERIALS AND METHODS: 103 human semen samples were evaluated using both Acridine Orange test and p53 ELISA and results were compared. RESULTS: A clear correlation between the values measured by two methods was obtained. CONCLUSIONS: If this hypothesis will be confirmed by further studies, the p53 ELISA assay could become a new and more precise indicator of DNA damage in human spermatozoa. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4229798/ /pubmed/25395748 http://dx.doi.org/10.4103/0974-1208.142490 Text en Copyright: © Journal of Human Reproductive Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Raimondo, Salvatore Gentile, Tommaso Cuomo, Felice De Filippo, Stefania Aprea, Gilda E. Guida, John Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
title | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
title_full | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
title_fullStr | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
title_full_unstemmed | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
title_short | Quantitative evaluation of p53 as a new indicator of DNA damage in human spermatozoa |
title_sort | quantitative evaluation of p53 as a new indicator of dna damage in human spermatozoa |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229798/ https://www.ncbi.nlm.nih.gov/pubmed/25395748 http://dx.doi.org/10.4103/0974-1208.142490 |
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