Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells

Fluorescence tagging of proteins is a widely used tool to study protein function and dynamics in live cells. However, the extent to which different mammalian transgene methods faithfully report on the properties of endogenous proteins has not been studied comparatively. Here we use quantitative live...

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Detalles Bibliográficos
Autores principales: Mahen, Robert, Koch, Birgit, Wachsmuth, Malte, Politi, Antonio Z., Perez-Gonzalez, Alexis, Mergenthaler, Julia, Cai, Yin, Ellenberg, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230620/
https://www.ncbi.nlm.nih.gov/pubmed/25232003
http://dx.doi.org/10.1091/mbc.E14-06-1091

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230620/
https://www.ncbi.nlm.nih.gov/pubmed/25232003
http://dx.doi.org/10.1091/mbc.E14-06-1091

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