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Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells
Fluorescence tagging of proteins is a widely used tool to study protein function and dynamics in live cells. However, the extent to which different mammalian transgene methods faithfully report on the properties of endogenous proteins has not been studied comparatively. Here we use quantitative live...
Autores principales: | Mahen, Robert, Koch, Birgit, Wachsmuth, Malte, Politi, Antonio Z., Perez-Gonzalez, Alexis, Mergenthaler, Julia, Cai, Yin, Ellenberg, Jan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230620/ https://www.ncbi.nlm.nih.gov/pubmed/25232003 http://dx.doi.org/10.1091/mbc.E14-06-1091 |
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