A High-Yield Co-Expression System for the Purification of an Intact Drs2p-Cdc50p Lipid Flippase Complex, Critically Dependent on and Stabilized by Phosphatidylinositol-4-Phosphate

P-type ATPases from the P4 subfamily (P4-ATPases) are energy-dependent transporters, which are thought to establish lipid asymmetry in eukaryotic cell membranes. Together with their Cdc50 accessory subunits, P4-ATPases couple ATP hydrolysis to lipid transport from the exoplasmic to the cytoplasmic l...

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Detalles Bibliográficos
Autores principales: Azouaoui, Hassina, Montigny, Cédric, Ash, Miriam-Rose, Fijalkowski, Frank, Jacquot, Aurore, Grønberg, Christina, López-Marqués, Rosa L., Palmgren, Michael G., Garrigos, Manuel, le Maire, Marc, Decottignies, Paulette, Gourdon, Pontus, Nissen, Poul, Champeil, Philippe, Lenoir, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230938/
https://www.ncbi.nlm.nih.gov/pubmed/25393116
http://dx.doi.org/10.1371/journal.pone.0112176

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