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CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis

Knockout of genes with CRISPR/Cas9 is a newly emerged approach to investigate functions of genes in various organisms. We demonstrate that CRISPR/Cas9 can mutate endogenous genes of the ascidian Ciona intestinalis, a splendid model for elucidating molecular mechanisms for constructing the chordate b...

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Detalles Bibliográficos
Autores principales:	Sasaki, Haruka, Yoshida, Keita, Hozumi, Akiko, Sasakura, Yasunori
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BlackWell Publishing Ltd 2014
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231237/ https://www.ncbi.nlm.nih.gov/pubmed/25212715 http://dx.doi.org/10.1111/dgd.12149

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