Cargando…

Quantitative determination of decitabine incorporation into DNA and its effect on mutation rates in human cancer cells

Decitabine (5-aza-2′-deoxycytidine) is a DNA methyltransferase inhibitor and an archetypal epigenetic drug for the therapy of myeloid leukemias. The mode of action of decitabine strictly depends on the incorporation of the drug into DNA. However, DNA incorporation and ensuing genotoxic effects of de...

Descripción completa

Detalles Bibliográficos
Autores principales:	Öz, Simin, Raddatz, Günter, Rius, Maria, Blagitko-Dorfs, Nadja, Lübbert, Michael, Maercker, Christian, Lyko, Frank
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231731/ https://www.ncbi.nlm.nih.gov/pubmed/25159616 http://dx.doi.org/10.1093/nar/gku775

Ejemplares similares

RNA cytosine methylation analysis by bisulfite sequencing
por: Schaefer, Matthias, et al.
Publicado: (2009)

Characterization of in vitro and in vivo hypomethylating effects of decitabine in acute myeloid leukemia by a rapid, specific and sensitive LC-MS/MS method
por: Liu, Zhongfa, et al.
Publicado: (2007)

Array-based analysis of genomic DNA methylation patterns of the tumour suppressor gene p16(INK4A) promoter in colon carcinoma cell lines
por: Mund, Cora, et al.
Publicado: (2005)

Incorporating cell hierarchy to decipher the functional diversity of single cells
por: Chen, Lingxi, et al.
Publicado: (2022)

Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation (BATI)
por: Zhou, Guo-Hua, et al.
Publicado: (2005)

Incorporating sequence quality data into alignment improves DNA read mapping
por: Frith, Martin C., et al.
Publicado: (2010)

Detection of base analogs incorporated during DNA replication by nanopore sequencing
por: Georgieva, Daniela, et al.
Publicado: (2020)

Epigenetic Priming of AML Blasts for All-trans Retinoic Acid-Induced Differentiation by the HDAC Class-I Selective Inhibitor Entinostat
por: Blagitko-Dorfs, Nadja, et al.
Publicado: (2013)

Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2′-deoxyuridine
por: Hua, Hui, et al.
Publicado: (2011)

Rationally evolving tRNA(Pyl) for efficient incorporation of noncanonical amino acids
por: Fan, Chenguang, et al.
Publicado: (2015)

Quantitative telomeric overhang determination using a double-strand specific nuclease
por: Zhao, Yong, et al.
Publicado: (2008)

Integrative study of EZH2 mutational status, copy number, protein expression and H3K27 trimethylation in AML/MDS patients
por: Stomper, Julia, et al.
Publicado: (2021)

Electrospray ionisation-cleavable tandem nucleic acid mass tag–peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS
por: Thompson, Andrew, et al.
Publicado: (2007)

Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates
por: Matovina, Mihaela, et al.
Publicado: (2010)

A powerful test for multiple rare variants association studies that incorporates sequencing qualities
por: Daye, Z. John, et al.
Publicado: (2012)

Correction to: Integrative study of EZH2 mutational status, copy number, protein expression and H3K27 trimethylation in AML/MDS patients
por: Stomper, Julia, et al.
Publicado: (2021)

A one-step method for quantitative determination of uracil in DNA by real-time PCR
por: Horváth, András, et al.
Publicado: (2010)

Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy
por: Walsh, Matthew T., et al.
Publicado: (2017)

Incorporation of aptamers in the terminal loop of shRNAs yields an effective and novel combinatorial targeting strategy
por: Pang, Ka Ming, et al.
Publicado: (2018)

Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution
por: Ludlow, Andrew T., et al.
Publicado: (2014)

cn.MOPS: mixture of Poissons for discovering copy number variations in next-generation sequencing data with a low false discovery rate
por: Klambauer, Günter, et al.
Publicado: (2012)

Enhanced and prolonged baculovirus-mediated expression by incorporating recombinase system and in cis elements: a comparative study
por: Sung, Li-Yu, et al.
Publicado: (2013)

Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells
por: Bartoschek, Michael D, et al.
Publicado: (2021)

Measurable impact of RNA quality on gene expression results from quantitative PCR
por: Vermeulen, Joëlle, et al.
Publicado: (2011)

Quantitation of cellular deoxynucleoside triphosphates
por: Ferraro, Paola, et al.
Publicado: (2010)

cn.FARMS: a latent variable model to detect copy number variations in microarray data with a low false discovery rate
por: Clevert, Djork-Arné, et al.
Publicado: (2011)

Nanoliter high throughput quantitative PCR
por: Morrison, Tom, et al.
Publicado: (2006)

Benchmarking spliced alignment programs including Spaln2, an extended version of Spaln that incorporates additional species-specific features
por: Iwata, Hiroaki, et al.
Publicado: (2012)

Novel enzymatic single-nucleotide modification of DNA oligomer: prevention of incessant incorporation of nucleotidyl transferase by ribonucleotide-borate complex
por: Jang, Eui Kyoung, et al.
Publicado: (2019)

External oligonucleotide standards enable cross laboratory comparison and exchange of real-time quantitative PCR data
por: Vermeulen, Joëlle, et al.
Publicado: (2009)

A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU)
por: Limsirichaikul, Siripan, et al.
Publicado: (2009)

Rapid quantitative profiling of complex microbial populations
por: Palmer, Chana, et al.
Publicado: (2006)

Detection of alternative lengthening of telomeres by telomere quantitative PCR
por: Lau, Loretta M. S., et al.
Publicado: (2013)

Absolute quantitative measurement of transcriptional kinetic parameters in vivo
por: Iyer, Sukanya, et al.
Publicado: (2016)

Generation of a conditionally self-eliminating HAC gene delivery vector through incorporation of a tTA(VP64) expression cassette
por: Kononenko, Artem V., et al.
Publicado: (2015)

TaqMan probe array for quantitative detection of DNA targets
por: Liu, Heping, et al.
Publicado: (2006)

A large field CCD system for quantitative imaging of microarrays
por: Hamilton, G., et al.
Publicado: (2006)

Zip nucleic acids are potent hydrolysis probes for quantitative PCR
por: Paris, Clément, et al.
Publicado: (2010)

X-inactivation: quantitative predictions of protein interactions in the Xist network
por: Agostini, Federico, et al.
Publicado: (2013)

Quantitative and simultaneous translational control of distinct mammalian mRNAs
por: Endo, Kei, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_7idpb3pt7jh3bkdvses7ct3jl1