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Mycobacterium avium genotype is associated with the therapeutic response to lung infection

Factors that can interfere with the successful treatment of Mycobacterium avium lung infection have been inadequately studied. To identify a potent predictor of therapeutic responses of M. avium lung infection, we analyzed variable number tandem repeats (VNTR) at 16 minisatellite loci of M. avium cl...

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Autores principales: Kikuchi, T, Kobashi, Y, Hirano, T, Tode, N, Santoso, A, Tamada, T, Fujimura, S, Mitsuhashi, Y, Honda, Y, Nukiwa, T, Kaku, M, Watanabe, A, Ichinose, M, Drancourt, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231998/
https://www.ncbi.nlm.nih.gov/pubmed/23829301
http://dx.doi.org/10.1111/1469-0691.12285
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author Kikuchi, T
Kobashi, Y
Hirano, T
Tode, N
Santoso, A
Tamada, T
Fujimura, S
Mitsuhashi, Y
Honda, Y
Nukiwa, T
Kaku, M
Watanabe, A
Ichinose, M
Drancourt, M
author_facet Kikuchi, T
Kobashi, Y
Hirano, T
Tode, N
Santoso, A
Tamada, T
Fujimura, S
Mitsuhashi, Y
Honda, Y
Nukiwa, T
Kaku, M
Watanabe, A
Ichinose, M
Drancourt, M
author_sort Kikuchi, T
collection PubMed
description Factors that can interfere with the successful treatment of Mycobacterium avium lung infection have been inadequately studied. To identify a potent predictor of therapeutic responses of M. avium lung infection, we analyzed variable number tandem repeats (VNTR) at 16 minisatellite loci of M. avium clinical isolates. Associations between the VNTR profiling data and a therapeutic response were evaluated in 59 subjects with M. avium lung infection. M. avium lung infection of 30 subjects in whom clarithromycin-containing regimens produced microbiological and radiographic improvement was defined as responsive disease, while that of the remaining 29 subjects was defined as refractory disease. In phylogenetic analysis using the genotypic distance aggregated from 16-dimensional VNTR data, 59 M. avium isolates were divided into three clusters, which showed a nearly significant association with therapeutic responses (p 0.06). We then subjected the raw 16-dimensional VNTR data directly to principal component analysis, and identified the genetic features that were significantly associated with the therapeutic response (p <0.05). By further analysis of logistic regression with a stepwise variable-selection, we constructed the highest likelihood multivariate model, adjusted for age, to predict a therapeutic response, using VNTR data from only four minisatellite loci. In conclusion, we identified four mycobacterial minisatellite loci that together were associated with the therapeutic response of M. avium lung infections.
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spelling pubmed-42319982014-12-19 Mycobacterium avium genotype is associated with the therapeutic response to lung infection Kikuchi, T Kobashi, Y Hirano, T Tode, N Santoso, A Tamada, T Fujimura, S Mitsuhashi, Y Honda, Y Nukiwa, T Kaku, M Watanabe, A Ichinose, M Drancourt, M Clin Microbiol Infect Bacteriology Factors that can interfere with the successful treatment of Mycobacterium avium lung infection have been inadequately studied. To identify a potent predictor of therapeutic responses of M. avium lung infection, we analyzed variable number tandem repeats (VNTR) at 16 minisatellite loci of M. avium clinical isolates. Associations between the VNTR profiling data and a therapeutic response were evaluated in 59 subjects with M. avium lung infection. M. avium lung infection of 30 subjects in whom clarithromycin-containing regimens produced microbiological and radiographic improvement was defined as responsive disease, while that of the remaining 29 subjects was defined as refractory disease. In phylogenetic analysis using the genotypic distance aggregated from 16-dimensional VNTR data, 59 M. avium isolates were divided into three clusters, which showed a nearly significant association with therapeutic responses (p 0.06). We then subjected the raw 16-dimensional VNTR data directly to principal component analysis, and identified the genetic features that were significantly associated with the therapeutic response (p <0.05). By further analysis of logistic regression with a stepwise variable-selection, we constructed the highest likelihood multivariate model, adjusted for age, to predict a therapeutic response, using VNTR data from only four minisatellite loci. In conclusion, we identified four mycobacterial minisatellite loci that together were associated with the therapeutic response of M. avium lung infections. BlackWell Publishing Ltd 2014-03 2013-07-05 /pmc/articles/PMC4231998/ /pubmed/23829301 http://dx.doi.org/10.1111/1469-0691.12285 Text en © 2013 The Authors Clinical Microbiology and Infection © 2013 European Society of Clinical Microbiology and Infectious Diseases http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Bacteriology
Kikuchi, T
Kobashi, Y
Hirano, T
Tode, N
Santoso, A
Tamada, T
Fujimura, S
Mitsuhashi, Y
Honda, Y
Nukiwa, T
Kaku, M
Watanabe, A
Ichinose, M
Drancourt, M
Mycobacterium avium genotype is associated with the therapeutic response to lung infection
title Mycobacterium avium genotype is associated with the therapeutic response to lung infection
title_full Mycobacterium avium genotype is associated with the therapeutic response to lung infection
title_fullStr Mycobacterium avium genotype is associated with the therapeutic response to lung infection
title_full_unstemmed Mycobacterium avium genotype is associated with the therapeutic response to lung infection
title_short Mycobacterium avium genotype is associated with the therapeutic response to lung infection
title_sort mycobacterium avium genotype is associated with the therapeutic response to lung infection
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231998/
https://www.ncbi.nlm.nih.gov/pubmed/23829301
http://dx.doi.org/10.1111/1469-0691.12285
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