ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes

The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleat...

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Detalles Bibliográficos
Autores principales: Gannon, Joan, Fernandez-Rodriguez, Julia, Alamri, Hussam, Feng, Shi Bo, Kalantari, Fariba, Negi, Sarita, Wong, Amy H. Y., Mazur, Alexander, Asp, Lennart, Fazel, Ali, Salman, Ayat, Lazaris, Anthoula, Metrakos, Peter, Bergeron, John J. M., Nilsson, Tommy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232254/
https://www.ncbi.nlm.nih.gov/pubmed/25397679
http://dx.doi.org/10.1371/journal.pone.0111309
Descripción
Sumario:The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleate in cultured cells. Also, that addition of cyclic AMP shifts ARFGAP1 from lipid droplets to the Golgi apparatus and that overexpression and knockdown of ARFGAP1 affect lipid droplet formation. Examination of human liver tissue reveals that ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes.

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