ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes

The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleat...

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Autores principales: Gannon, Joan, Fernandez-Rodriguez, Julia, Alamri, Hussam, Feng, Shi Bo, Kalantari, Fariba, Negi, Sarita, Wong, Amy H. Y., Mazur, Alexander, Asp, Lennart, Fazel, Ali, Salman, Ayat, Lazaris, Anthoula, Metrakos, Peter, Bergeron, John J. M., Nilsson, Tommy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232254/
https://www.ncbi.nlm.nih.gov/pubmed/25397679
http://dx.doi.org/10.1371/journal.pone.0111309
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author Gannon, Joan
Fernandez-Rodriguez, Julia
Alamri, Hussam
Feng, Shi Bo
Kalantari, Fariba
Negi, Sarita
Wong, Amy H. Y.
Mazur, Alexander
Asp, Lennart
Fazel, Ali
Salman, Ayat
Lazaris, Anthoula
Metrakos, Peter
Bergeron, John J. M.
Nilsson, Tommy
author_facet Gannon, Joan
Fernandez-Rodriguez, Julia
Alamri, Hussam
Feng, Shi Bo
Kalantari, Fariba
Negi, Sarita
Wong, Amy H. Y.
Mazur, Alexander
Asp, Lennart
Fazel, Ali
Salman, Ayat
Lazaris, Anthoula
Metrakos, Peter
Bergeron, John J. M.
Nilsson, Tommy
author_sort Gannon, Joan
collection PubMed
description The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleate in cultured cells. Also, that addition of cyclic AMP shifts ARFGAP1 from lipid droplets to the Golgi apparatus and that overexpression and knockdown of ARFGAP1 affect lipid droplet formation. Examination of human liver tissue reveals that ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes.
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spelling pubmed-42322542014-11-26 ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes Gannon, Joan Fernandez-Rodriguez, Julia Alamri, Hussam Feng, Shi Bo Kalantari, Fariba Negi, Sarita Wong, Amy H. Y. Mazur, Alexander Asp, Lennart Fazel, Ali Salman, Ayat Lazaris, Anthoula Metrakos, Peter Bergeron, John J. M. Nilsson, Tommy PLoS One Research Article The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleate in cultured cells. Also, that addition of cyclic AMP shifts ARFGAP1 from lipid droplets to the Golgi apparatus and that overexpression and knockdown of ARFGAP1 affect lipid droplet formation. Examination of human liver tissue reveals that ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes. Public Library of Science 2014-11-14 /pmc/articles/PMC4232254/ /pubmed/25397679 http://dx.doi.org/10.1371/journal.pone.0111309 Text en © 2014 Gannon et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gannon, Joan
Fernandez-Rodriguez, Julia
Alamri, Hussam
Feng, Shi Bo
Kalantari, Fariba
Negi, Sarita
Wong, Amy H. Y.
Mazur, Alexander
Asp, Lennart
Fazel, Ali
Salman, Ayat
Lazaris, Anthoula
Metrakos, Peter
Bergeron, John J. M.
Nilsson, Tommy
ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes
title ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes
title_full ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes
title_fullStr ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes
title_full_unstemmed ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes
title_short ARFGAP1 Is Dynamically Associated with Lipid Droplets in Hepatocytes
title_sort arfgap1 is dynamically associated with lipid droplets in hepatocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232254/
https://www.ncbi.nlm.nih.gov/pubmed/25397679
http://dx.doi.org/10.1371/journal.pone.0111309
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