The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance

Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain an...

Descripción completa

Detalles Bibliográficos
Autores principales: Hyun, Tae Kyung, van der Graaff, Eric, Albacete, Alfonso, Eom, Seung Hee, Großkinsky, Dominik K., Böhm, Hannah, Janschek, Ursula, Rim, Yeonggil, Ali, Walid Wahid, Kim, Soo Young, Roitsch, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232524/
https://www.ncbi.nlm.nih.gov/pubmed/25396746
http://dx.doi.org/10.1371/journal.pone.0112946
_version_ 1782344579428646912
author Hyun, Tae Kyung
van der Graaff, Eric
Albacete, Alfonso
Eom, Seung Hee
Großkinsky, Dominik K.
Böhm, Hannah
Janschek, Ursula
Rim, Yeonggil
Ali, Walid Wahid
Kim, Soo Young
Roitsch, Thomas
author_facet Hyun, Tae Kyung
van der Graaff, Eric
Albacete, Alfonso
Eom, Seung Hee
Großkinsky, Dominik K.
Böhm, Hannah
Janschek, Ursula
Rim, Yeonggil
Ali, Walid Wahid
Kim, Soo Young
Roitsch, Thomas
author_sort Hyun, Tae Kyung
collection PubMed
description Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty.
format Online
Article
Text
id pubmed-4232524
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-42325242014-11-26 The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance Hyun, Tae Kyung van der Graaff, Eric Albacete, Alfonso Eom, Seung Hee Großkinsky, Dominik K. Böhm, Hannah Janschek, Ursula Rim, Yeonggil Ali, Walid Wahid Kim, Soo Young Roitsch, Thomas PLoS One Research Article Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty. Public Library of Science 2014-11-14 /pmc/articles/PMC4232524/ /pubmed/25396746 http://dx.doi.org/10.1371/journal.pone.0112946 Text en © 2014 Hyun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hyun, Tae Kyung
van der Graaff, Eric
Albacete, Alfonso
Eom, Seung Hee
Großkinsky, Dominik K.
Böhm, Hannah
Janschek, Ursula
Rim, Yeonggil
Ali, Walid Wahid
Kim, Soo Young
Roitsch, Thomas
The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance
title The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance
title_full The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance
title_fullStr The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance
title_full_unstemmed The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance
title_short The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance
title_sort arabidopsis plat domain protein1 is critically involved in abiotic stress tolerance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232524/
https://www.ncbi.nlm.nih.gov/pubmed/25396746
http://dx.doi.org/10.1371/journal.pone.0112946
work_keys_str_mv AT hyuntaekyung thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT vandergraafferic thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT albacetealfonso thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT eomseunghee thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT großkinskydominikk thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT bohmhannah thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT janschekursula thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT rimyeonggil thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT aliwalidwahid thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT kimsooyoung thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT roitschthomas thearabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT hyuntaekyung arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT vandergraafferic arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT albacetealfonso arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT eomseunghee arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT großkinskydominikk arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT bohmhannah arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT janschekursula arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT rimyeonggil arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT aliwalidwahid arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT kimsooyoung arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance
AT roitschthomas arabidopsisplatdomainprotein1iscriticallyinvolvedinabioticstresstolerance