The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance
Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain an...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232524/ https://www.ncbi.nlm.nih.gov/pubmed/25396746 http://dx.doi.org/10.1371/journal.pone.0112946 |
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author | Hyun, Tae Kyung van der Graaff, Eric Albacete, Alfonso Eom, Seung Hee Großkinsky, Dominik K. Böhm, Hannah Janschek, Ursula Rim, Yeonggil Ali, Walid Wahid Kim, Soo Young Roitsch, Thomas |
author_facet | Hyun, Tae Kyung van der Graaff, Eric Albacete, Alfonso Eom, Seung Hee Großkinsky, Dominik K. Böhm, Hannah Janschek, Ursula Rim, Yeonggil Ali, Walid Wahid Kim, Soo Young Roitsch, Thomas |
author_sort | Hyun, Tae Kyung |
collection | PubMed |
description | Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty. |
format | Online Article Text |
id | pubmed-4232524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42325242014-11-26 The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance Hyun, Tae Kyung van der Graaff, Eric Albacete, Alfonso Eom, Seung Hee Großkinsky, Dominik K. Böhm, Hannah Janschek, Ursula Rim, Yeonggil Ali, Walid Wahid Kim, Soo Young Roitsch, Thomas PLoS One Research Article Despite the completion of the Arabidopsis genome sequence, for only a relatively low percentage of the encoded proteins experimental evidence concerning their function is available. Plant proteins that harbour a single PLAT (Polycystin, Lipoxygenase, Alpha-toxin and Triacylglycerol lipase) domain and belong to the PLAT-plant-stress protein family are ubiquitously present in monocot and dicots. However, the function of PLAT-plant-stress proteins is still poorly understood. Therefore, we have assessed the function of the uncharacterised Arabidopsis PLAT-plant-stress family members through a combination of functional genetic and physiological approaches. PLAT1 overexpression conferred increased abiotic stress tolerance, including cold, drought and salt stress, while loss-of-function resulted in opposite effects on abiotic stress tolerance. Strikingly, PLAT1 promoted growth under non-stressed conditions. Abiotic stress treatments induced PLAT1 expression and caused expansion of its expression domain. The ABF/ABRE transcription factors, which are positive mediators of abscisic acid signalling, activate PLAT1 promoter activity in transactivation assays and directly bind to the ABRE elements located in this promoter in electrophoretic mobility shift assays. This suggests that PLAT1 represents a novel downstream target of the abscisic acid signalling pathway. Thus, we showed that PLAT1 critically functions as positive regulator of abiotic stress tolerance, but also is involved in regulating plant growth, and thereby assigned a function to this previously uncharacterised PLAT domain protein. The functional data obtained for PLAT1 support that PLAT-plant-stress proteins in general could be promising targets for improving abiotic stress tolerance without yield penalty. Public Library of Science 2014-11-14 /pmc/articles/PMC4232524/ /pubmed/25396746 http://dx.doi.org/10.1371/journal.pone.0112946 Text en © 2014 Hyun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hyun, Tae Kyung van der Graaff, Eric Albacete, Alfonso Eom, Seung Hee Großkinsky, Dominik K. Böhm, Hannah Janschek, Ursula Rim, Yeonggil Ali, Walid Wahid Kim, Soo Young Roitsch, Thomas The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance |
title | The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance |
title_full | The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance |
title_fullStr | The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance |
title_full_unstemmed | The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance |
title_short | The Arabidopsis PLAT Domain Protein1 Is Critically Involved in Abiotic Stress Tolerance |
title_sort | arabidopsis plat domain protein1 is critically involved in abiotic stress tolerance |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232524/ https://www.ncbi.nlm.nih.gov/pubmed/25396746 http://dx.doi.org/10.1371/journal.pone.0112946 |
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