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Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila
The CRISPR-associated RNA-guided nuclease Cas9 has emerged as a powerful tool for genome engineering in a variety of organisms. To achieve efficient gene targeting rates in Drosophila, current approaches require either injection of in vitro transcribed RNAs or injection into transgenic Cas9-expressi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232553/ https://www.ncbi.nlm.nih.gov/pubmed/25236734 http://dx.doi.org/10.1534/g3.114.014126 |