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Recent advances in hydrogen peroxide imaging for biological applications

Mounting evidence supports the role of hydrogen peroxide (H(2)O(2)) in physiological signaling as well as pathological conditions. However, the subtleties of peroxide-mediated signaling are not well understood, in part because the generation, degradation, and diffusion of H(2)O(2) are highly volatil...

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Autores principales: Guo, Hengchang, Aleyasin, Hossein, Dickinson, Bryan C, Haskew-Layton, Renée E, Ratan, Rajiv R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232666/
https://www.ncbi.nlm.nih.gov/pubmed/25400906
http://dx.doi.org/10.1186/2045-3701-4-64
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author Guo, Hengchang
Aleyasin, Hossein
Dickinson, Bryan C
Haskew-Layton, Renée E
Ratan, Rajiv R
author_facet Guo, Hengchang
Aleyasin, Hossein
Dickinson, Bryan C
Haskew-Layton, Renée E
Ratan, Rajiv R
author_sort Guo, Hengchang
collection PubMed
description Mounting evidence supports the role of hydrogen peroxide (H(2)O(2)) in physiological signaling as well as pathological conditions. However, the subtleties of peroxide-mediated signaling are not well understood, in part because the generation, degradation, and diffusion of H(2)O(2) are highly volatile within different cellular compartments. Therefore, the direct measurement of H(2)O(2) in living specimens is critically important. Fluorescent probes that can detect small changes in H(2)O(2) levels within relevant cellular compartments are important tools to study the spatial dynamics of H(2)O(2). To achieve temporal resolution, the probes must also be photostable enough to allow multiple readings over time without loss of signal. Traditional fluorescent redox sensitive probes that have been commonly used for the detection of H(2)O(2) tend to react with a wide variety of reactive oxygen species (ROS) and often suffer from photostablilty issues. Recently, new classes of H(2)O(2) probes have been designed to detect H(2)O(2) with high selectivity. Advances in H(2)O(2) measurement have enabled biomedical scientists to study H(2)O(2) biology at a level of precision previously unachievable. In addition, new imaging techniques such as two-photon microscopy (TPM) have been employed for H(2)O(2) detection, which permit real-time measurements of H(2)O(2)in vivo. This review focuses on recent advances in H(2)O(2) probe development and optical imaging technologies that have been developed for biomedical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2045-3701-4-64) contains supplementary material, which is available to authorized users.
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spelling pubmed-42326662014-11-16 Recent advances in hydrogen peroxide imaging for biological applications Guo, Hengchang Aleyasin, Hossein Dickinson, Bryan C Haskew-Layton, Renée E Ratan, Rajiv R Cell Biosci Review Mounting evidence supports the role of hydrogen peroxide (H(2)O(2)) in physiological signaling as well as pathological conditions. However, the subtleties of peroxide-mediated signaling are not well understood, in part because the generation, degradation, and diffusion of H(2)O(2) are highly volatile within different cellular compartments. Therefore, the direct measurement of H(2)O(2) in living specimens is critically important. Fluorescent probes that can detect small changes in H(2)O(2) levels within relevant cellular compartments are important tools to study the spatial dynamics of H(2)O(2). To achieve temporal resolution, the probes must also be photostable enough to allow multiple readings over time without loss of signal. Traditional fluorescent redox sensitive probes that have been commonly used for the detection of H(2)O(2) tend to react with a wide variety of reactive oxygen species (ROS) and often suffer from photostablilty issues. Recently, new classes of H(2)O(2) probes have been designed to detect H(2)O(2) with high selectivity. Advances in H(2)O(2) measurement have enabled biomedical scientists to study H(2)O(2) biology at a level of precision previously unachievable. In addition, new imaging techniques such as two-photon microscopy (TPM) have been employed for H(2)O(2) detection, which permit real-time measurements of H(2)O(2)in vivo. This review focuses on recent advances in H(2)O(2) probe development and optical imaging technologies that have been developed for biomedical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/2045-3701-4-64) contains supplementary material, which is available to authorized users. BioMed Central 2014-10-27 /pmc/articles/PMC4232666/ /pubmed/25400906 http://dx.doi.org/10.1186/2045-3701-4-64 Text en © Guo et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Guo, Hengchang
Aleyasin, Hossein
Dickinson, Bryan C
Haskew-Layton, Renée E
Ratan, Rajiv R
Recent advances in hydrogen peroxide imaging for biological applications
title Recent advances in hydrogen peroxide imaging for biological applications
title_full Recent advances in hydrogen peroxide imaging for biological applications
title_fullStr Recent advances in hydrogen peroxide imaging for biological applications
title_full_unstemmed Recent advances in hydrogen peroxide imaging for biological applications
title_short Recent advances in hydrogen peroxide imaging for biological applications
title_sort recent advances in hydrogen peroxide imaging for biological applications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232666/
https://www.ncbi.nlm.nih.gov/pubmed/25400906
http://dx.doi.org/10.1186/2045-3701-4-64
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