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The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties
In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO(3))(2) (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dip...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232794/ https://www.ncbi.nlm.nih.gov/pubmed/25587317 |
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author | Shahraki, Somaye Mansouri-Torshizi, Hassan Sori Nezami, Ziba Ghahghaei, Arezou Yaghoubi, Fatemeh Divsalar, Adeleh Saboury, Ali-Akbar H. Shirazi, Farshad |
author_facet | Shahraki, Somaye Mansouri-Torshizi, Hassan Sori Nezami, Ziba Ghahghaei, Arezou Yaghoubi, Fatemeh Divsalar, Adeleh Saboury, Ali-Akbar H. Shirazi, Farshad |
author_sort | Shahraki, Somaye |
collection | PubMed |
description | In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO(3))(2) (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have been investigated by utilizing the electronic absorption spectroscopy, fluorescence spectra and ethidium bromide (EBr) displacement and gel filtration techniques. a-d complexes cooperatively bind and denature the DNA at low concentrations. Their concentration at midpoint of transition, L(1/2), follows the order a >> b > c > d. Also the g, the number of binding sites per 1000 nucleotides, follows the order a >> b ~ c > d. EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a. Several binding and thermodynamic parameters are also described. The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc(50)) values much lower than cisplatin. |
format | Online Article Text |
id | pubmed-4232794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-42327942015-01-13 The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties Shahraki, Somaye Mansouri-Torshizi, Hassan Sori Nezami, Ziba Ghahghaei, Arezou Yaghoubi, Fatemeh Divsalar, Adeleh Saboury, Ali-Akbar H. Shirazi, Farshad Iran J Pharm Res Original Article In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO(3))(2) (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have been investigated by utilizing the electronic absorption spectroscopy, fluorescence spectra and ethidium bromide (EBr) displacement and gel filtration techniques. a-d complexes cooperatively bind and denature the DNA at low concentrations. Their concentration at midpoint of transition, L(1/2), follows the order a >> b > c > d. Also the g, the number of binding sites per 1000 nucleotides, follows the order a >> b ~ c > d. EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a. Several binding and thermodynamic parameters are also described. The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc(50)) values much lower than cisplatin. Shaheed Beheshti University of Medical Sciences 2014 /pmc/articles/PMC4232794/ /pubmed/25587317 Text en © 2014 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Shahraki, Somaye Mansouri-Torshizi, Hassan Sori Nezami, Ziba Ghahghaei, Arezou Yaghoubi, Fatemeh Divsalar, Adeleh Saboury, Ali-Akbar H. Shirazi, Farshad The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties |
title | The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties |
title_full | The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties |
title_fullStr | The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties |
title_full_unstemmed | The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties |
title_short | The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties |
title_sort | effects of extending of co-planarity in a series of structurally relative polypyridyl palladium(ii) complexes on dna-binding and cytotoxicity properties |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232794/ https://www.ncbi.nlm.nih.gov/pubmed/25587317 |
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