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Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs
BACKGROUND: Brain and immune system are linked in a bi-directional manner. To date, it remained largely unknown why immune components become suppressed, enhanced, or remain unaffected in relation to psychosocial stress. Therefore, we mixed unfamiliar pigs with different levels of aggressiveness. We...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4233077/ https://www.ncbi.nlm.nih.gov/pubmed/25380980 http://dx.doi.org/10.1186/1471-2164-15-967 |
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author | Oster, Michael Muráni, Eduard Ponsuksili, Siriluck D’Eath, Richard B Turner, Simon P Evans, Gary Thölking, Ludger Kurt, Esra Klont, Ronald Foury, Aline Mormède, Pierre Wimmers, Klaus |
author_facet | Oster, Michael Muráni, Eduard Ponsuksili, Siriluck D’Eath, Richard B Turner, Simon P Evans, Gary Thölking, Ludger Kurt, Esra Klont, Ronald Foury, Aline Mormède, Pierre Wimmers, Klaus |
author_sort | Oster, Michael |
collection | PubMed |
description | BACKGROUND: Brain and immune system are linked in a bi-directional manner. To date, it remained largely unknown why immune components become suppressed, enhanced, or remain unaffected in relation to psychosocial stress. Therefore, we mixed unfamiliar pigs with different levels of aggressiveness. We separated castrated male and female pigs into psychosocially high- and low- stressed animals by skin lesions, plasma cortisol level, and creatine kinase activity obtained from agonistic behaviour associated with regrouping. Peripheral blood mononuclear cells (PBMC) were collected post-mortem and differential gene expression was assessed using the Affymetrix platform (n = 16). RESULTS: Relevant stress-dependent alterations were found only between female samples, but not between castrated male samples. Molecular routes related to TREM 1 signalling, dendritic cell maturation, IL-6 signalling, Toll-like receptor signalling, and IL-8 signalling were increased in high stressed females compared to low stressed females. This indicates a launch of immune effector molecules as a direct response. According to the shifts of transcripts encoding cell surface receptors (e.g. CD14, TLR2, TLR4, TREM1) the study highlights processes acting on pattern recognition, inflammation, and cell-cell communication. CONCLUSIONS: The transcriptional response partly affected the degree of ‘stress responsiveness’, indicating that the high stressed females altered their signal transduction due to potential infections and injuries while fighting. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-967) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4233077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42330772014-11-17 Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs Oster, Michael Muráni, Eduard Ponsuksili, Siriluck D’Eath, Richard B Turner, Simon P Evans, Gary Thölking, Ludger Kurt, Esra Klont, Ronald Foury, Aline Mormède, Pierre Wimmers, Klaus BMC Genomics Research Article BACKGROUND: Brain and immune system are linked in a bi-directional manner. To date, it remained largely unknown why immune components become suppressed, enhanced, or remain unaffected in relation to psychosocial stress. Therefore, we mixed unfamiliar pigs with different levels of aggressiveness. We separated castrated male and female pigs into psychosocially high- and low- stressed animals by skin lesions, plasma cortisol level, and creatine kinase activity obtained from agonistic behaviour associated with regrouping. Peripheral blood mononuclear cells (PBMC) were collected post-mortem and differential gene expression was assessed using the Affymetrix platform (n = 16). RESULTS: Relevant stress-dependent alterations were found only between female samples, but not between castrated male samples. Molecular routes related to TREM 1 signalling, dendritic cell maturation, IL-6 signalling, Toll-like receptor signalling, and IL-8 signalling were increased in high stressed females compared to low stressed females. This indicates a launch of immune effector molecules as a direct response. According to the shifts of transcripts encoding cell surface receptors (e.g. CD14, TLR2, TLR4, TREM1) the study highlights processes acting on pattern recognition, inflammation, and cell-cell communication. CONCLUSIONS: The transcriptional response partly affected the degree of ‘stress responsiveness’, indicating that the high stressed females altered their signal transduction due to potential infections and injuries while fighting. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-967) contains supplementary material, which is available to authorized users. BioMed Central 2014-11-08 /pmc/articles/PMC4233077/ /pubmed/25380980 http://dx.doi.org/10.1186/1471-2164-15-967 Text en © Oster et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Oster, Michael Muráni, Eduard Ponsuksili, Siriluck D’Eath, Richard B Turner, Simon P Evans, Gary Thölking, Ludger Kurt, Esra Klont, Ronald Foury, Aline Mormède, Pierre Wimmers, Klaus Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs |
title | Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs |
title_full | Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs |
title_fullStr | Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs |
title_full_unstemmed | Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs |
title_short | Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs |
title_sort | transcriptional responses of pbmc in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4233077/ https://www.ncbi.nlm.nih.gov/pubmed/25380980 http://dx.doi.org/10.1186/1471-2164-15-967 |
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