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Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data
The analysis of single molecule imaging experiments is complicated by the stochastic nature of single molecule events, by instrument noise and by the limited information which can be gathered about any individual molecule observed. Consequently, it is important to cross check experimental results us...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Royal Society
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4233692/ https://www.ncbi.nlm.nih.gov/pubmed/25008080 http://dx.doi.org/10.1098/rsif.2014.0442 |
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author | Mashanov, Gregory I. |
author_facet | Mashanov, Gregory I. |
author_sort | Mashanov, Gregory I. |
collection | PubMed |
description | The analysis of single molecule imaging experiments is complicated by the stochastic nature of single molecule events, by instrument noise and by the limited information which can be gathered about any individual molecule observed. Consequently, it is important to cross check experimental results using a model simulating single molecule dynamics (e.g. movements and binding events) in a virtual cell-like environment. The output of such a model should match the real data format allowing researchers to compare simulated results with the real experiments. The proposed model exploits the advantages of ‘object-oriented’ computing. First of all, the ability to create and manipulate a number of classes, each containing an arbitrary number of single molecule objects. These classes may include objects moving within the ‘cytoplasm’; objects moving at the ‘plasma membrane’; and static objects located inside the ‘body’. The objects of a given class can interact with each other and/or with the objects of other classes according to their physical and chemical properties. Each model run generates a sequence of images, each containing summed images of all fluorescent objects emitting light under given illumination conditions with realistic levels of noise and emission fluctuations. The model accurately reproduces reported single molecule experiments and predicts the outcome of future experiments. |
format | Online Article Text |
id | pubmed-4233692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-42336922014-11-21 Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data Mashanov, Gregory I. J R Soc Interface Research Articles The analysis of single molecule imaging experiments is complicated by the stochastic nature of single molecule events, by instrument noise and by the limited information which can be gathered about any individual molecule observed. Consequently, it is important to cross check experimental results using a model simulating single molecule dynamics (e.g. movements and binding events) in a virtual cell-like environment. The output of such a model should match the real data format allowing researchers to compare simulated results with the real experiments. The proposed model exploits the advantages of ‘object-oriented’ computing. First of all, the ability to create and manipulate a number of classes, each containing an arbitrary number of single molecule objects. These classes may include objects moving within the ‘cytoplasm’; objects moving at the ‘plasma membrane’; and static objects located inside the ‘body’. The objects of a given class can interact with each other and/or with the objects of other classes according to their physical and chemical properties. Each model run generates a sequence of images, each containing summed images of all fluorescent objects emitting light under given illumination conditions with realistic levels of noise and emission fluctuations. The model accurately reproduces reported single molecule experiments and predicts the outcome of future experiments. The Royal Society 2014-09-06 /pmc/articles/PMC4233692/ /pubmed/25008080 http://dx.doi.org/10.1098/rsif.2014.0442 Text en http://creativecommons.org/licenses/by/3.0/ © 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Articles Mashanov, Gregory I. Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data |
title | Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data |
title_full | Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data |
title_fullStr | Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data |
title_full_unstemmed | Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data |
title_short | Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data |
title_sort | single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4233692/ https://www.ncbi.nlm.nih.gov/pubmed/25008080 http://dx.doi.org/10.1098/rsif.2014.0442 |
work_keys_str_mv | AT mashanovgregoryi singlemoleculedynamicsinavirtualcellathreedimensionalmodelthatproducessimulatedfluorescencevideoimagingdata |