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Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas

OBJECTIVE: The purposes of this study were to histologically assess different types of oral squamous cell carcinoma and the silver-binding nucleolar organizer region (AgNOR) morphology in neoplastic cells, as well as to quantify the number of AgNORs in each type of carcinoma in order to relate AgNOR...

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Autores principales: HANEMANN, João Adolfo Costa, MIYAZAWA, Marta, SOUZA, Mireile São Geraldo dos Santos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculdade de Odontologia de Bauru da Universidade de São Paulo 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234343/
https://www.ncbi.nlm.nih.gov/pubmed/21625747
http://dx.doi.org/10.1590/S1678-77572011000300018
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author HANEMANN, João Adolfo Costa
MIYAZAWA, Marta
SOUZA, Mireile São Geraldo dos Santos
author_facet HANEMANN, João Adolfo Costa
MIYAZAWA, Marta
SOUZA, Mireile São Geraldo dos Santos
author_sort HANEMANN, João Adolfo Costa
collection PubMed
description OBJECTIVE: The purposes of this study were to histologically assess different types of oral squamous cell carcinoma and the silver-binding nucleolar organizer region (AgNOR) morphology in neoplastic cells, as well as to quantify the number of AgNORs in each type of carcinoma in order to relate AgNOR count and histologic grading. MATERIAL AND METHODS: Twenty-eight cases of oral squamous cell carcinoma were divided into 4 groups, namely well-differentiated, moderately differentiated, poorly differentiated, and undifferentiated. For NOR study, 3-µm-thick sections were stained with 50% aqueous silver nitrate solution. The predominant microscopic pattern of NORs was determined. Quantitative analyses of NORs were obtained of all cells present on each histological field using a 0.025 mm(2) eyepiece graticule. Different histological fields were analyzed until the total number of NORs was 120 cells for each tumor. Kruskall-Wallis test was applied to compare the groups of sample data at a significance level of p=0.05. RESULTS: The mean number of AgNORs per nucleus was 3.20 for the well-differentiated group, 5.33 for the moderately differentiated one, 8.27 for the poorly differentiated one, and 10.08 for the undifferentiated one. AgNOR count was significantly different (p<0.05) among all of the studied groups. CONCLUSION: AgNOR staining technique seems to be a useful diagnostic tool since differences in AgNOR numeric values can be identified in the different types of oral squamous cell carcinoma. This technique is easy to handle and inexpensive, thus justifying its large use in histopathology.
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spelling pubmed-42343432014-11-19 Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas HANEMANN, João Adolfo Costa MIYAZAWA, Marta SOUZA, Mireile São Geraldo dos Santos J Appl Oral Sci Original Articles OBJECTIVE: The purposes of this study were to histologically assess different types of oral squamous cell carcinoma and the silver-binding nucleolar organizer region (AgNOR) morphology in neoplastic cells, as well as to quantify the number of AgNORs in each type of carcinoma in order to relate AgNOR count and histologic grading. MATERIAL AND METHODS: Twenty-eight cases of oral squamous cell carcinoma were divided into 4 groups, namely well-differentiated, moderately differentiated, poorly differentiated, and undifferentiated. For NOR study, 3-µm-thick sections were stained with 50% aqueous silver nitrate solution. The predominant microscopic pattern of NORs was determined. Quantitative analyses of NORs were obtained of all cells present on each histological field using a 0.025 mm(2) eyepiece graticule. Different histological fields were analyzed until the total number of NORs was 120 cells for each tumor. Kruskall-Wallis test was applied to compare the groups of sample data at a significance level of p=0.05. RESULTS: The mean number of AgNORs per nucleus was 3.20 for the well-differentiated group, 5.33 for the moderately differentiated one, 8.27 for the poorly differentiated one, and 10.08 for the undifferentiated one. AgNOR count was significantly different (p<0.05) among all of the studied groups. CONCLUSION: AgNOR staining technique seems to be a useful diagnostic tool since differences in AgNOR numeric values can be identified in the different types of oral squamous cell carcinoma. This technique is easy to handle and inexpensive, thus justifying its large use in histopathology. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2011 /pmc/articles/PMC4234343/ /pubmed/21625747 http://dx.doi.org/10.1590/S1678-77572011000300018 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
HANEMANN, João Adolfo Costa
MIYAZAWA, Marta
SOUZA, Mireile São Geraldo dos Santos
Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas
title Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas
title_full Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas
title_fullStr Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas
title_full_unstemmed Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas
title_short Histologic grading and nucleolar organizer regions in oral squamous cell carcinomas
title_sort histologic grading and nucleolar organizer regions in oral squamous cell carcinomas
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234343/
https://www.ncbi.nlm.nih.gov/pubmed/21625747
http://dx.doi.org/10.1590/S1678-77572011000300018
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