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Effect of reduced exposure times on the cytotoxicity of resin luting cements cured by high-power led
OBJECTIVE: Applications of resin luting agents and high-power light-emitting diodes (LED) light-curing units (LCUs) have increased considerably over the last few years. However, it is not clear whether the effect of reduced exposure time on cytotoxicity of such products have adequate biocompatibilit...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade de Odontologia de Bauru da Universidade de São
Paulo
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234344/ https://www.ncbi.nlm.nih.gov/pubmed/21625748 http://dx.doi.org/10.1590/S1678-77572011000300019 |
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author | ERGUN, Gulfem EGILMEZ, Ferhan YILMAZ, Sukran |
author_facet | ERGUN, Gulfem EGILMEZ, Ferhan YILMAZ, Sukran |
author_sort | ERGUN, Gulfem |
collection | PubMed |
description | OBJECTIVE: Applications of resin luting agents and high-power light-emitting diodes (LED) light-curing units (LCUs) have increased considerably over the last few years. However, it is not clear whether the effect of reduced exposure time on cytotoxicity of such products have adequate biocompatibility to meet clinical success. This study aimed at assessing the effect of reduced curing time of five resin luting cements (RLCs) polymerized by high-power LED curing unit on the viability of a cell of L-929 fibroblast cells. MATERIAL AND METHODS: Disc-shaped samples were prepared in polytetrafluoroethylene moulds with cylindrical cavities. The samples were irradiated from the top through the ceramic discs and acetate strips using LED LCU for 20 s (50% of the manufacturer's recommended exposure time) and 40 s (100% exposure time). After curing, the samples were transferred into a culture medium for 24 h. The eluates were obtained and pipetted onto L-929 fibroblast cultures (3x10(4) per well) and incubated for evaluating after 24 h. Measurements were performed by dimethylthiazol diphenyltetrazolium assay. Statistical significance was determined by two-way ANOVA and two independent samples were compared by t-test. RESULTS: Results showed that eluates of most of the materials polymerized for 20 s (except Rely X Unicem and Illusion) reduced to a higher extent cell viability compared to samples of the same materials polymerized for 40 s. Illusion exhibited the least cytotoxicity for 20 s exposure time compared to the control (culture without samples) followed by Rely X Unicem and Rely X ARC (90.81%, 88.90%, and 83.11%, respectively). For Rely X ARC, Duolink and Lute-It 40 s exposure time was better (t=-1.262 p=0,276; t=-9.399 p=0.001; and t=-20.418 p<0.001, respectively). CONCLUSION: The results of this study suggest that reduction of curing time significantly enhances the cytotoxicity of the studied resin cement materials, therefore compromising their clinical performance. |
format | Online Article Text |
id | pubmed-4234344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Faculdade de Odontologia de Bauru da Universidade de São
Paulo |
record_format | MEDLINE/PubMed |
spelling | pubmed-42343442014-11-19 Effect of reduced exposure times on the cytotoxicity of resin luting cements cured by high-power led ERGUN, Gulfem EGILMEZ, Ferhan YILMAZ, Sukran J Appl Oral Sci Original Articles OBJECTIVE: Applications of resin luting agents and high-power light-emitting diodes (LED) light-curing units (LCUs) have increased considerably over the last few years. However, it is not clear whether the effect of reduced exposure time on cytotoxicity of such products have adequate biocompatibility to meet clinical success. This study aimed at assessing the effect of reduced curing time of five resin luting cements (RLCs) polymerized by high-power LED curing unit on the viability of a cell of L-929 fibroblast cells. MATERIAL AND METHODS: Disc-shaped samples were prepared in polytetrafluoroethylene moulds with cylindrical cavities. The samples were irradiated from the top through the ceramic discs and acetate strips using LED LCU for 20 s (50% of the manufacturer's recommended exposure time) and 40 s (100% exposure time). After curing, the samples were transferred into a culture medium for 24 h. The eluates were obtained and pipetted onto L-929 fibroblast cultures (3x10(4) per well) and incubated for evaluating after 24 h. Measurements were performed by dimethylthiazol diphenyltetrazolium assay. Statistical significance was determined by two-way ANOVA and two independent samples were compared by t-test. RESULTS: Results showed that eluates of most of the materials polymerized for 20 s (except Rely X Unicem and Illusion) reduced to a higher extent cell viability compared to samples of the same materials polymerized for 40 s. Illusion exhibited the least cytotoxicity for 20 s exposure time compared to the control (culture without samples) followed by Rely X Unicem and Rely X ARC (90.81%, 88.90%, and 83.11%, respectively). For Rely X ARC, Duolink and Lute-It 40 s exposure time was better (t=-1.262 p=0,276; t=-9.399 p=0.001; and t=-20.418 p<0.001, respectively). CONCLUSION: The results of this study suggest that reduction of curing time significantly enhances the cytotoxicity of the studied resin cement materials, therefore compromising their clinical performance. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2011 /pmc/articles/PMC4234344/ /pubmed/21625748 http://dx.doi.org/10.1590/S1678-77572011000300019 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles ERGUN, Gulfem EGILMEZ, Ferhan YILMAZ, Sukran Effect of reduced exposure times on the cytotoxicity of resin luting cements cured by high-power led |
title | Effect of reduced exposure times on the cytotoxicity of resin luting
cements cured by high-power led |
title_full | Effect of reduced exposure times on the cytotoxicity of resin luting
cements cured by high-power led |
title_fullStr | Effect of reduced exposure times on the cytotoxicity of resin luting
cements cured by high-power led |
title_full_unstemmed | Effect of reduced exposure times on the cytotoxicity of resin luting
cements cured by high-power led |
title_short | Effect of reduced exposure times on the cytotoxicity of resin luting
cements cured by high-power led |
title_sort | effect of reduced exposure times on the cytotoxicity of resin luting
cements cured by high-power led |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234344/ https://www.ncbi.nlm.nih.gov/pubmed/21625748 http://dx.doi.org/10.1590/S1678-77572011000300019 |
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