Cargando…
RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe
The eukaryotic small-subunit (SSU) ribosomal RNA (rRNA) has two evolutionarily conserved acetylcytidines. However, the acetylation sites and the acetyltransferase responsible for the acetylation have not been identified. We performed a comprehensive MS-based analysis covering the entire sequence of...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234376/ https://www.ncbi.nlm.nih.gov/pubmed/25402480 http://dx.doi.org/10.1371/journal.pone.0112156 |
_version_ | 1782344846243004416 |
---|---|
author | Taoka, Masato Ishikawa, Daisuke Nobe, Yuko Ishikawa, Hideaki Yamauchi, Yoshio Terukina, Goro Nakayama, Hiroshi Hirota, Kouji Takahashi, Nobuhiro Isobe, Toshiaki |
author_facet | Taoka, Masato Ishikawa, Daisuke Nobe, Yuko Ishikawa, Hideaki Yamauchi, Yoshio Terukina, Goro Nakayama, Hiroshi Hirota, Kouji Takahashi, Nobuhiro Isobe, Toshiaki |
author_sort | Taoka, Masato |
collection | PubMed |
description | The eukaryotic small-subunit (SSU) ribosomal RNA (rRNA) has two evolutionarily conserved acetylcytidines. However, the acetylation sites and the acetyltransferase responsible for the acetylation have not been identified. We performed a comprehensive MS-based analysis covering the entire sequence of the fission yeast, Schizosaccharomyces pombe, SSU rRNA and identified two acetylcytidines at positions 1297 and 1815 in the 3′ half of the rRNA. To identify the enzyme responsible for the cytidine acetylation, we searched for an S. pombe gene homologous to TmcA, a bacterial tRNA N-acetyltransferase, and found one potential candidate, Nat10. A temperature-sensitive strain of Nat10 with a mutation in the Walker A type ATP-binding motif abolished the cytidine acetylation in SSU rRNA, and the wild-type Nat10 supplemented to this strain recovered the acetylation, providing evidence that Nat10 is necessary for acetylation of SSU rRNA. The Nat10 mutant strain showed a slow-growth phenotype and was defective in forming the SSU rRNA from the precursor RNA, suggesting that cytidine acetylation is necessary for ribosome assembly. |
format | Online Article Text |
id | pubmed-4234376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42343762014-11-21 RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe Taoka, Masato Ishikawa, Daisuke Nobe, Yuko Ishikawa, Hideaki Yamauchi, Yoshio Terukina, Goro Nakayama, Hiroshi Hirota, Kouji Takahashi, Nobuhiro Isobe, Toshiaki PLoS One Research Article The eukaryotic small-subunit (SSU) ribosomal RNA (rRNA) has two evolutionarily conserved acetylcytidines. However, the acetylation sites and the acetyltransferase responsible for the acetylation have not been identified. We performed a comprehensive MS-based analysis covering the entire sequence of the fission yeast, Schizosaccharomyces pombe, SSU rRNA and identified two acetylcytidines at positions 1297 and 1815 in the 3′ half of the rRNA. To identify the enzyme responsible for the cytidine acetylation, we searched for an S. pombe gene homologous to TmcA, a bacterial tRNA N-acetyltransferase, and found one potential candidate, Nat10. A temperature-sensitive strain of Nat10 with a mutation in the Walker A type ATP-binding motif abolished the cytidine acetylation in SSU rRNA, and the wild-type Nat10 supplemented to this strain recovered the acetylation, providing evidence that Nat10 is necessary for acetylation of SSU rRNA. The Nat10 mutant strain showed a slow-growth phenotype and was defective in forming the SSU rRNA from the precursor RNA, suggesting that cytidine acetylation is necessary for ribosome assembly. Public Library of Science 2014-11-17 /pmc/articles/PMC4234376/ /pubmed/25402480 http://dx.doi.org/10.1371/journal.pone.0112156 Text en © 2014 Taoka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Taoka, Masato Ishikawa, Daisuke Nobe, Yuko Ishikawa, Hideaki Yamauchi, Yoshio Terukina, Goro Nakayama, Hiroshi Hirota, Kouji Takahashi, Nobuhiro Isobe, Toshiaki RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe |
title | RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe
|
title_full | RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe
|
title_fullStr | RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe
|
title_full_unstemmed | RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe
|
title_short | RNA Cytidine Acetyltransferase of Small-Subunit Ribosomal RNA: Identification of Acetylation Sites and the Responsible Acetyltransferase in Fission Yeast, Schizosaccharomyces pombe
|
title_sort | rna cytidine acetyltransferase of small-subunit ribosomal rna: identification of acetylation sites and the responsible acetyltransferase in fission yeast, schizosaccharomyces pombe |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234376/ https://www.ncbi.nlm.nih.gov/pubmed/25402480 http://dx.doi.org/10.1371/journal.pone.0112156 |
work_keys_str_mv | AT taokamasato rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT ishikawadaisuke rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT nobeyuko rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT ishikawahideaki rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT yamauchiyoshio rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT terukinagoro rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT nakayamahiroshi rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT hirotakouji rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT takahashinobuhiro rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe AT isobetoshiaki rnacytidineacetyltransferaseofsmallsubunitribosomalrnaidentificationofacetylationsitesandtheresponsibleacetyltransferaseinfissionyeastschizosaccharomycespombe |