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The intestinal microbiome of fish under starvation

BACKGROUND: Starvation not only affects the nutritional and health status of the animals, but also the microbial composition in the host’s intestine. Next-generation sequencing provides a unique opportunity to explore gut microbial communities and their interactions with hosts. However, studies on g...

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Autores principales: Xia, Jun Hong, Lin, Grace, Fu, Gui Hong, Wan, Zi Yi, Lee, May, Wang, Le, Liu, Xiao Jun, Yue, Gen Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234480/
https://www.ncbi.nlm.nih.gov/pubmed/24708260
http://dx.doi.org/10.1186/1471-2164-15-266
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author Xia, Jun Hong
Lin, Grace
Fu, Gui Hong
Wan, Zi Yi
Lee, May
Wang, Le
Liu, Xiao Jun
Yue, Gen Hua
author_facet Xia, Jun Hong
Lin, Grace
Fu, Gui Hong
Wan, Zi Yi
Lee, May
Wang, Le
Liu, Xiao Jun
Yue, Gen Hua
author_sort Xia, Jun Hong
collection PubMed
description BACKGROUND: Starvation not only affects the nutritional and health status of the animals, but also the microbial composition in the host’s intestine. Next-generation sequencing provides a unique opportunity to explore gut microbial communities and their interactions with hosts. However, studies on gut microbiomes have been conducted predominantly in humans and land animals. Not much is known on gut microbiomes of aquatic animals and their changes under changing environmental conditions. To address this shortcoming, we determined the microbial gene catalogue, and investigated changes in the microbial composition and host-microbe interactions in the intestine of Asian seabass in response to starvation. RESULTS: We found 33 phyla, 66 classes, 130 orders and 278 families in the intestinal microbiome. Proteobacteria (48.8%), Firmicutes (15.3%) and Bacteroidetes (8.2%) were the three most abundant bacteria taxa. Comparative analyses of the microbiome revealed shifts in bacteria communities, with dramatic enrichment of Bacteroidetes, but significant depletion of Betaproteobacteria in starved intestines. In addition, significant differences in clusters of orthologous groups (COG) functional categories and orthologous groups were observed. Genes related to antibiotic activity in the microbiome were significantly enriched in response to starvation, and host genes related to the immune response were generally up-regulated. CONCLUSIONS: This study provides the first insights into the fish intestinal microbiome and its changes under starvation. Further detailed study on interactions between intestinal microbiomes and hosts under dynamic conditions will shed new light on how the hosts and microbes respond to the changing environment.
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spelling pubmed-42344802014-11-18 The intestinal microbiome of fish under starvation Xia, Jun Hong Lin, Grace Fu, Gui Hong Wan, Zi Yi Lee, May Wang, Le Liu, Xiao Jun Yue, Gen Hua BMC Genomics Research Article BACKGROUND: Starvation not only affects the nutritional and health status of the animals, but also the microbial composition in the host’s intestine. Next-generation sequencing provides a unique opportunity to explore gut microbial communities and their interactions with hosts. However, studies on gut microbiomes have been conducted predominantly in humans and land animals. Not much is known on gut microbiomes of aquatic animals and their changes under changing environmental conditions. To address this shortcoming, we determined the microbial gene catalogue, and investigated changes in the microbial composition and host-microbe interactions in the intestine of Asian seabass in response to starvation. RESULTS: We found 33 phyla, 66 classes, 130 orders and 278 families in the intestinal microbiome. Proteobacteria (48.8%), Firmicutes (15.3%) and Bacteroidetes (8.2%) were the three most abundant bacteria taxa. Comparative analyses of the microbiome revealed shifts in bacteria communities, with dramatic enrichment of Bacteroidetes, but significant depletion of Betaproteobacteria in starved intestines. In addition, significant differences in clusters of orthologous groups (COG) functional categories and orthologous groups were observed. Genes related to antibiotic activity in the microbiome were significantly enriched in response to starvation, and host genes related to the immune response were generally up-regulated. CONCLUSIONS: This study provides the first insights into the fish intestinal microbiome and its changes under starvation. Further detailed study on interactions between intestinal microbiomes and hosts under dynamic conditions will shed new light on how the hosts and microbes respond to the changing environment. BioMed Central 2014-04-05 /pmc/articles/PMC4234480/ /pubmed/24708260 http://dx.doi.org/10.1186/1471-2164-15-266 Text en Copyright © 2014 Xia et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research Article
Xia, Jun Hong
Lin, Grace
Fu, Gui Hong
Wan, Zi Yi
Lee, May
Wang, Le
Liu, Xiao Jun
Yue, Gen Hua
The intestinal microbiome of fish under starvation
title The intestinal microbiome of fish under starvation
title_full The intestinal microbiome of fish under starvation
title_fullStr The intestinal microbiome of fish under starvation
title_full_unstemmed The intestinal microbiome of fish under starvation
title_short The intestinal microbiome of fish under starvation
title_sort intestinal microbiome of fish under starvation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234480/
https://www.ncbi.nlm.nih.gov/pubmed/24708260
http://dx.doi.org/10.1186/1471-2164-15-266
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