Cargando…
Characterization of Treponema denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC
Treponema denticola, a gram-negative and anaerobic spirochete, is associated with advancing severity of chronic periodontitis. In this study, we confirmed that two major antigenic proteinswere Msp and TmpC, and examined their physiological and pathological roles using gene-deletion mutants. Msp form...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234677/ https://www.ncbi.nlm.nih.gov/pubmed/25401769 http://dx.doi.org/10.1371/journal.pone.0113565 |
_version_ | 1782344894016126976 |
---|---|
author | Abiko, Yuki Nagano, Keiji Yoshida, Yasuo Yoshimura, Fuminobu |
author_facet | Abiko, Yuki Nagano, Keiji Yoshida, Yasuo Yoshimura, Fuminobu |
author_sort | Abiko, Yuki |
collection | PubMed |
description | Treponema denticola, a gram-negative and anaerobic spirochete, is associated with advancing severity of chronic periodontitis. In this study, we confirmed that two major antigenic proteinswere Msp and TmpC, and examined their physiological and pathological roles using gene-deletion mutants. Msp formed a large complex that localized to the outer membrane, while TmpC existed as a monomer and largely localized to the inner membrane. However, TmpC was also detected in the outer membrane fraction, but its cell-surface exposure was not detected. Msp defects increased cell-surface hydrophobicity and secretion of TNF-α from macrophage-like cells, whereas TmpC defects decreased autoagglutination and chymotrypsin-like protease activities. Both mutants adhered to gingival epithelial cells similarly to the wild-type and showed slightly decreased motility. In addition, in Msp-defective mutants, the TDE1072 protein, which is a major membrane protein, was abolished; therefore, phenotypic changes in the mutant can be, at least in part, attributed to the loss of the TDE1072 protein. Thus, the major antigenic proteins, Msp and TmpC, have significant and diverse impacts on the characteristics of T. denticola, especially cell surface properties. |
format | Online Article Text |
id | pubmed-4234677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42346772014-11-21 Characterization of Treponema denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC Abiko, Yuki Nagano, Keiji Yoshida, Yasuo Yoshimura, Fuminobu PLoS One Research Article Treponema denticola, a gram-negative and anaerobic spirochete, is associated with advancing severity of chronic periodontitis. In this study, we confirmed that two major antigenic proteinswere Msp and TmpC, and examined their physiological and pathological roles using gene-deletion mutants. Msp formed a large complex that localized to the outer membrane, while TmpC existed as a monomer and largely localized to the inner membrane. However, TmpC was also detected in the outer membrane fraction, but its cell-surface exposure was not detected. Msp defects increased cell-surface hydrophobicity and secretion of TNF-α from macrophage-like cells, whereas TmpC defects decreased autoagglutination and chymotrypsin-like protease activities. Both mutants adhered to gingival epithelial cells similarly to the wild-type and showed slightly decreased motility. In addition, in Msp-defective mutants, the TDE1072 protein, which is a major membrane protein, was abolished; therefore, phenotypic changes in the mutant can be, at least in part, attributed to the loss of the TDE1072 protein. Thus, the major antigenic proteins, Msp and TmpC, have significant and diverse impacts on the characteristics of T. denticola, especially cell surface properties. Public Library of Science 2014-11-17 /pmc/articles/PMC4234677/ /pubmed/25401769 http://dx.doi.org/10.1371/journal.pone.0113565 Text en © 2014 Abiko et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Abiko, Yuki Nagano, Keiji Yoshida, Yasuo Yoshimura, Fuminobu Characterization of Treponema denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC |
title | Characterization of Treponema denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC |
title_full | Characterization of Treponema denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC |
title_fullStr | Characterization of Treponema denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC |
title_full_unstemmed | Characterization of Treponema denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC |
title_short | Characterization of Treponema denticola Mutants Defective in the Major Antigenic Proteins, Msp and TmpC |
title_sort | characterization of treponema denticola mutants defective in the major antigenic proteins, msp and tmpc |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234677/ https://www.ncbi.nlm.nih.gov/pubmed/25401769 http://dx.doi.org/10.1371/journal.pone.0113565 |
work_keys_str_mv | AT abikoyuki characterizationoftreponemadenticolamutantsdefectiveinthemajorantigenicproteinsmspandtmpc AT naganokeiji characterizationoftreponemadenticolamutantsdefectiveinthemajorantigenicproteinsmspandtmpc AT yoshidayasuo characterizationoftreponemadenticolamutantsdefectiveinthemajorantigenicproteinsmspandtmpc AT yoshimurafuminobu characterizationoftreponemadenticolamutantsdefectiveinthemajorantigenicproteinsmspandtmpc |