Cargando…

Comparability of hemoglobin A1c level measured in capillary versus venous blood sample applying two point-of-care instruments

BACKGROUND: The present study is designed to evaluate the validity of the measurement of capillary blood hemoglobin A1c levels in comparison with venous blood hemoglobin A1c. METHODS: The data of this cross-sectional study are collected from a sample of 45 Iranian diabetic patients referred to one p...

Descripción completa

Detalles Bibliográficos
Autores principales: Keramati, Tahereh, Razi, Farideh, Tootee, Ali, Larijani, Bagher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234901/
https://www.ncbi.nlm.nih.gov/pubmed/25408924
http://dx.doi.org/10.1186/s40200-014-0094-1
_version_ 1782344932938219520
author Keramati, Tahereh
Razi, Farideh
Tootee, Ali
Larijani, Bagher
author_facet Keramati, Tahereh
Razi, Farideh
Tootee, Ali
Larijani, Bagher
author_sort Keramati, Tahereh
collection PubMed
description BACKGROUND: The present study is designed to evaluate the validity of the measurement of capillary blood hemoglobin A1c levels in comparison with venous blood hemoglobin A1c. METHODS: The data of this cross-sectional study are collected from a sample of 45 Iranian diabetic patients referred to one particular laboratory for the assessment of HbA1c level during a period from April to December 2013. Venous and simultaneous capillary blood samples were obtained from each subject for measurement of hemoglobin A1c levels. Both samples were tested using two different NGSP certified systems: CERA STAT 2000 (Ceragem Medisys Inc) and NycoCard Reader II (Axis-Shield). RESULTS: The mean hemoglobin A1c in venous and capillary blood samples measured using CERA STAT 2000 assays were 6.30 ± 1.68% and 6.34 ± 1.65% respectively (p = 0.590). However, when NycoCard Reader II assay was employed, the mean hemoglobin A1c in venous and capillary blood samples were 6.73 ± 1.35% and 6.92 ± 1.50% (p = 0.007). Moreover, a strong correlation was observed between venous and capillary hemoglobin A1c levels with Pearson’s concordance correlation coefficients of 0.96 and 0.94 with the use of NycoCard Reader II and CERA STAT 2000 assays respectively. Application of CERA STAT 2000 demonstrated to be of a considerably higher value using the ROC curve analysis assay (AUC = 0.991). Also, similar analysis by using NycoCard Reader II assay demonstrated that capillary hemoglobin A1c measurement had high value for differentiation of uncontrolled from controlled blood glucose level (AUC = 0.935). CONCLUSION: It was demonstrated that capillary hemoglobin A1c measurement had a considerably high value for differentiating between poorly-controlled and well-controlled blood glucose levels.
format Online
Article
Text
id pubmed-4234901
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-42349012014-11-19 Comparability of hemoglobin A1c level measured in capillary versus venous blood sample applying two point-of-care instruments Keramati, Tahereh Razi, Farideh Tootee, Ali Larijani, Bagher J Diabetes Metab Disord Research Article BACKGROUND: The present study is designed to evaluate the validity of the measurement of capillary blood hemoglobin A1c levels in comparison with venous blood hemoglobin A1c. METHODS: The data of this cross-sectional study are collected from a sample of 45 Iranian diabetic patients referred to one particular laboratory for the assessment of HbA1c level during a period from April to December 2013. Venous and simultaneous capillary blood samples were obtained from each subject for measurement of hemoglobin A1c levels. Both samples were tested using two different NGSP certified systems: CERA STAT 2000 (Ceragem Medisys Inc) and NycoCard Reader II (Axis-Shield). RESULTS: The mean hemoglobin A1c in venous and capillary blood samples measured using CERA STAT 2000 assays were 6.30 ± 1.68% and 6.34 ± 1.65% respectively (p = 0.590). However, when NycoCard Reader II assay was employed, the mean hemoglobin A1c in venous and capillary blood samples were 6.73 ± 1.35% and 6.92 ± 1.50% (p = 0.007). Moreover, a strong correlation was observed between venous and capillary hemoglobin A1c levels with Pearson’s concordance correlation coefficients of 0.96 and 0.94 with the use of NycoCard Reader II and CERA STAT 2000 assays respectively. Application of CERA STAT 2000 demonstrated to be of a considerably higher value using the ROC curve analysis assay (AUC = 0.991). Also, similar analysis by using NycoCard Reader II assay demonstrated that capillary hemoglobin A1c measurement had high value for differentiation of uncontrolled from controlled blood glucose level (AUC = 0.935). CONCLUSION: It was demonstrated that capillary hemoglobin A1c measurement had a considerably high value for differentiating between poorly-controlled and well-controlled blood glucose levels. BioMed Central 2014-10-30 /pmc/articles/PMC4234901/ /pubmed/25408924 http://dx.doi.org/10.1186/s40200-014-0094-1 Text en © Keramati et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Keramati, Tahereh
Razi, Farideh
Tootee, Ali
Larijani, Bagher
Comparability of hemoglobin A1c level measured in capillary versus venous blood sample applying two point-of-care instruments
title Comparability of hemoglobin A1c level measured in capillary versus venous blood sample applying two point-of-care instruments
title_full Comparability of hemoglobin A1c level measured in capillary versus venous blood sample applying two point-of-care instruments
title_fullStr Comparability of hemoglobin A1c level measured in capillary versus venous blood sample applying two point-of-care instruments
title_full_unstemmed Comparability of hemoglobin A1c level measured in capillary versus venous blood sample applying two point-of-care instruments
title_short Comparability of hemoglobin A1c level measured in capillary versus venous blood sample applying two point-of-care instruments
title_sort comparability of hemoglobin a1c level measured in capillary versus venous blood sample applying two point-of-care instruments
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234901/
https://www.ncbi.nlm.nih.gov/pubmed/25408924
http://dx.doi.org/10.1186/s40200-014-0094-1
work_keys_str_mv AT keramatitahereh comparabilityofhemoglobina1clevelmeasuredincapillaryversusvenousbloodsampleapplyingtwopointofcareinstruments
AT razifarideh comparabilityofhemoglobina1clevelmeasuredincapillaryversusvenousbloodsampleapplyingtwopointofcareinstruments
AT tooteeali comparabilityofhemoglobina1clevelmeasuredincapillaryversusvenousbloodsampleapplyingtwopointofcareinstruments
AT larijanibagher comparabilityofhemoglobina1clevelmeasuredincapillaryversusvenousbloodsampleapplyingtwopointofcareinstruments