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The genome of the Lactobacillus sanfranciscensis temperate phage EV3

BACKGROUND: Bacteriophages infection modulates microbial consortia and transduction is one of the most important mechanism involved in the bacterial evolution. However, phage contamination brings food fermentations to a halt causing economic setbacks. The number of phage genome sequences of lactic a...

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Autores principales: Ehrmann, Matthias A, Angelov, Angel, Picozzi, Claudia, Foschino, Roberto, Vogel, Rudi F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234937/
https://www.ncbi.nlm.nih.gov/pubmed/24308641
http://dx.doi.org/10.1186/1756-0500-6-514
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author Ehrmann, Matthias A
Angelov, Angel
Picozzi, Claudia
Foschino, Roberto
Vogel, Rudi F
author_facet Ehrmann, Matthias A
Angelov, Angel
Picozzi, Claudia
Foschino, Roberto
Vogel, Rudi F
author_sort Ehrmann, Matthias A
collection PubMed
description BACKGROUND: Bacteriophages infection modulates microbial consortia and transduction is one of the most important mechanism involved in the bacterial evolution. However, phage contamination brings food fermentations to a halt causing economic setbacks. The number of phage genome sequences of lactic acid bacteria especially of lactobacilli is still limited. We analysed the genome of a temperate phage active on Lactobacillus sanfranciscensis, the predominant strain in type I sourdough fermentations. RESULTS: Sequencing of the DNA of EV3 phage revealed a genome of 34,834 bp and a G + C content of 36.45%. Of the 43 open reading frames (ORFs) identified, all but eight shared homology with other phages of lactobacilli. A similar genomic organization and mosaic pattern of identities align EV3 with the closely related Lactobacillus vaginalis ATCC 49540 prophage. Four unknown ORFs that had no homologies in the databases or predicted functions were identified. Notably, EV3 encodes a putative dextranase. CONCLUSIONS: EV3 is the first L. sanfranciscensis phage that has been completely sequenced so far.
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spelling pubmed-42349372014-11-19 The genome of the Lactobacillus sanfranciscensis temperate phage EV3 Ehrmann, Matthias A Angelov, Angel Picozzi, Claudia Foschino, Roberto Vogel, Rudi F BMC Res Notes Research Article BACKGROUND: Bacteriophages infection modulates microbial consortia and transduction is one of the most important mechanism involved in the bacterial evolution. However, phage contamination brings food fermentations to a halt causing economic setbacks. The number of phage genome sequences of lactic acid bacteria especially of lactobacilli is still limited. We analysed the genome of a temperate phage active on Lactobacillus sanfranciscensis, the predominant strain in type I sourdough fermentations. RESULTS: Sequencing of the DNA of EV3 phage revealed a genome of 34,834 bp and a G + C content of 36.45%. Of the 43 open reading frames (ORFs) identified, all but eight shared homology with other phages of lactobacilli. A similar genomic organization and mosaic pattern of identities align EV3 with the closely related Lactobacillus vaginalis ATCC 49540 prophage. Four unknown ORFs that had no homologies in the databases or predicted functions were identified. Notably, EV3 encodes a putative dextranase. CONCLUSIONS: EV3 is the first L. sanfranciscensis phage that has been completely sequenced so far. BioMed Central 2013-12-05 /pmc/articles/PMC4234937/ /pubmed/24308641 http://dx.doi.org/10.1186/1756-0500-6-514 Text en Copyright © 2013 Ehrmann et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ehrmann, Matthias A
Angelov, Angel
Picozzi, Claudia
Foschino, Roberto
Vogel, Rudi F
The genome of the Lactobacillus sanfranciscensis temperate phage EV3
title The genome of the Lactobacillus sanfranciscensis temperate phage EV3
title_full The genome of the Lactobacillus sanfranciscensis temperate phage EV3
title_fullStr The genome of the Lactobacillus sanfranciscensis temperate phage EV3
title_full_unstemmed The genome of the Lactobacillus sanfranciscensis temperate phage EV3
title_short The genome of the Lactobacillus sanfranciscensis temperate phage EV3
title_sort genome of the lactobacillus sanfranciscensis temperate phage ev3
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234937/
https://www.ncbi.nlm.nih.gov/pubmed/24308641
http://dx.doi.org/10.1186/1756-0500-6-514
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