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miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway
MicroRNAs (miRNAs) are important regulators of stem and progenitor cell functions. We previously reported that miR-142 and miR-150 are upregulated in human breast cancer stem cells (BCSCs) as compared to the non-tumorigenic breast cancer cells. In this study, we report that miR-142 efficiently recru...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235011/ https://www.ncbi.nlm.nih.gov/pubmed/25406066 http://dx.doi.org/10.7554/eLife.01977 |
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author | Isobe, Taichi Hisamori, Shigeo Hogan, Daniel J Zabala, Maider Hendrickson, David G Dalerba, Piero Cai, Shang Scheeren, Ferenc Kuo, Angera H Sikandar, Shaheen S Lam, Jessica S Qian, Dalong Dirbas, Frederick M Somlo, George Lao, Kaiqin Brown, Patrick O Clarke, Michael F Shimono, Yohei |
author_facet | Isobe, Taichi Hisamori, Shigeo Hogan, Daniel J Zabala, Maider Hendrickson, David G Dalerba, Piero Cai, Shang Scheeren, Ferenc Kuo, Angera H Sikandar, Shaheen S Lam, Jessica S Qian, Dalong Dirbas, Frederick M Somlo, George Lao, Kaiqin Brown, Patrick O Clarke, Michael F Shimono, Yohei |
author_sort | Isobe, Taichi |
collection | PubMed |
description | MicroRNAs (miRNAs) are important regulators of stem and progenitor cell functions. We previously reported that miR-142 and miR-150 are upregulated in human breast cancer stem cells (BCSCs) as compared to the non-tumorigenic breast cancer cells. In this study, we report that miR-142 efficiently recruits the APC mRNA to an RNA-induced silencing complex, activates the canonical WNT signaling pathway in an APC-suppression dependent manner, and activates the expression of miR-150. Enforced expression of miR-142 or miR-150 in normal mouse mammary stem cells resulted in the regeneration of hyperproliferative mammary glands in vivo. Knockdown of endogenous miR-142 effectively suppressed organoid formation by BCSCs and slowed tumor growth initiated by human BCSCs in vivo. These results suggest that in some tumors, miR-142 regulates the properties of BCSCs at least in part by activating the WNT signaling pathway and miR-150 expression. DOI: http://dx.doi.org/10.7554/eLife.01977.001 |
format | Online Article Text |
id | pubmed-4235011 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42350112014-11-21 miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway Isobe, Taichi Hisamori, Shigeo Hogan, Daniel J Zabala, Maider Hendrickson, David G Dalerba, Piero Cai, Shang Scheeren, Ferenc Kuo, Angera H Sikandar, Shaheen S Lam, Jessica S Qian, Dalong Dirbas, Frederick M Somlo, George Lao, Kaiqin Brown, Patrick O Clarke, Michael F Shimono, Yohei eLife Cell Biology MicroRNAs (miRNAs) are important regulators of stem and progenitor cell functions. We previously reported that miR-142 and miR-150 are upregulated in human breast cancer stem cells (BCSCs) as compared to the non-tumorigenic breast cancer cells. In this study, we report that miR-142 efficiently recruits the APC mRNA to an RNA-induced silencing complex, activates the canonical WNT signaling pathway in an APC-suppression dependent manner, and activates the expression of miR-150. Enforced expression of miR-142 or miR-150 in normal mouse mammary stem cells resulted in the regeneration of hyperproliferative mammary glands in vivo. Knockdown of endogenous miR-142 effectively suppressed organoid formation by BCSCs and slowed tumor growth initiated by human BCSCs in vivo. These results suggest that in some tumors, miR-142 regulates the properties of BCSCs at least in part by activating the WNT signaling pathway and miR-150 expression. DOI: http://dx.doi.org/10.7554/eLife.01977.001 eLife Sciences Publications, Ltd 2014-11-18 /pmc/articles/PMC4235011/ /pubmed/25406066 http://dx.doi.org/10.7554/eLife.01977 Text en Copyright © 2014, Isobe et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Isobe, Taichi Hisamori, Shigeo Hogan, Daniel J Zabala, Maider Hendrickson, David G Dalerba, Piero Cai, Shang Scheeren, Ferenc Kuo, Angera H Sikandar, Shaheen S Lam, Jessica S Qian, Dalong Dirbas, Frederick M Somlo, George Lao, Kaiqin Brown, Patrick O Clarke, Michael F Shimono, Yohei miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway |
title | miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway |
title_full | miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway |
title_fullStr | miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway |
title_full_unstemmed | miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway |
title_short | miR-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical WNT signaling pathway |
title_sort | mir-142 regulates the tumorigenicity of human breast cancer stem cells through the canonical wnt signaling pathway |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235011/ https://www.ncbi.nlm.nih.gov/pubmed/25406066 http://dx.doi.org/10.7554/eLife.01977 |
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