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Use of the Meganuclease I-SceI of Saccharomyces cerevisiae to select for gene deletions in actinomycetes
The search for new natural products is leading to the isolation of novel actinomycete species, many of which will ultimately require genetic analysis. Some of these isolates will likely exhibit low intrinsic frequencies of homologous recombination and fail to sporulate under laboratory conditions, e...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235310/ https://www.ncbi.nlm.nih.gov/pubmed/25403842 http://dx.doi.org/10.1038/srep07100 |