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Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus
Common marmosets (Callithrix jacchus) developed high levels of viremia, clinical signs including fever, weight loss, a decrease in activity and hematuria upon inoculation with dengue virus (DENV). Presence of DENV genome in urine samples and pathological changes in kidneys were examined in the prese...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235723/ https://www.ncbi.nlm.nih.gov/pubmed/25437039 http://dx.doi.org/10.3390/pathogens2020357 |
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author | Moi, Meng Ling Omatsu, Tsutomu Hirayama, Takanori Nakamura, Shinichiro Katakai, Yuko Yoshida, Tomoyuki Saito, Akatsuki Tajima, Shigeru Ito, Mikako Takasaki, Tomohiko Akari, Hirofumi Kurane, Ichiro |
author_facet | Moi, Meng Ling Omatsu, Tsutomu Hirayama, Takanori Nakamura, Shinichiro Katakai, Yuko Yoshida, Tomoyuki Saito, Akatsuki Tajima, Shigeru Ito, Mikako Takasaki, Tomohiko Akari, Hirofumi Kurane, Ichiro |
author_sort | Moi, Meng Ling |
collection | PubMed |
description | Common marmosets (Callithrix jacchus) developed high levels of viremia, clinical signs including fever, weight loss, a decrease in activity and hematuria upon inoculation with dengue virus (DENV). Presence of DENV genome in urine samples and pathological changes in kidneys were examined in the present study. Levels of DENV genome were determined in 228 urine samples from 20 primary DENV-inoculated marmosets and in 56 urine samples from four secondary DENV-inoculated marmosets. DENV genome was detected in 75% (15/20) of marmosets after primary DENV infection. No DENV genome was detected in urine samples from the marmosets with secondary infection with homologous DENV (0%, 0/4). Two marmosets demonstrated hematuria. Pathological analysis of the kidneys demonstrated non-suppressive interstitial nephritis with renal tubular regeneration. DENV antigen-positive cells were detected in kidneys. In human dengue virus infections, some patients present renal symptoms. The results indicate that marmosets recapitulate some aspects of the involvement of kidneys in human DENV infection, and suggest that marmosets are potentially useful for the studies of the pathogenesis of DENV infection, including kidneys. |
format | Online Article Text |
id | pubmed-4235723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-42357232014-11-25 Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus Moi, Meng Ling Omatsu, Tsutomu Hirayama, Takanori Nakamura, Shinichiro Katakai, Yuko Yoshida, Tomoyuki Saito, Akatsuki Tajima, Shigeru Ito, Mikako Takasaki, Tomohiko Akari, Hirofumi Kurane, Ichiro Pathogens Article Common marmosets (Callithrix jacchus) developed high levels of viremia, clinical signs including fever, weight loss, a decrease in activity and hematuria upon inoculation with dengue virus (DENV). Presence of DENV genome in urine samples and pathological changes in kidneys were examined in the present study. Levels of DENV genome were determined in 228 urine samples from 20 primary DENV-inoculated marmosets and in 56 urine samples from four secondary DENV-inoculated marmosets. DENV genome was detected in 75% (15/20) of marmosets after primary DENV infection. No DENV genome was detected in urine samples from the marmosets with secondary infection with homologous DENV (0%, 0/4). Two marmosets demonstrated hematuria. Pathological analysis of the kidneys demonstrated non-suppressive interstitial nephritis with renal tubular regeneration. DENV antigen-positive cells were detected in kidneys. In human dengue virus infections, some patients present renal symptoms. The results indicate that marmosets recapitulate some aspects of the involvement of kidneys in human DENV infection, and suggest that marmosets are potentially useful for the studies of the pathogenesis of DENV infection, including kidneys. MDPI 2013-05-13 /pmc/articles/PMC4235723/ /pubmed/25437039 http://dx.doi.org/10.3390/pathogens2020357 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Moi, Meng Ling Omatsu, Tsutomu Hirayama, Takanori Nakamura, Shinichiro Katakai, Yuko Yoshida, Tomoyuki Saito, Akatsuki Tajima, Shigeru Ito, Mikako Takasaki, Tomohiko Akari, Hirofumi Kurane, Ichiro Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus |
title | Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus |
title_full | Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus |
title_fullStr | Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus |
title_full_unstemmed | Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus |
title_short | Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus |
title_sort | presence of viral genome in urine and development of hematuria and pathological changes in kidneys in common marmoset (callithrix jacchus) after inoculation with dengue virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235723/ https://www.ncbi.nlm.nih.gov/pubmed/25437039 http://dx.doi.org/10.3390/pathogens2020357 |
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