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Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants

Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these pl...

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Autores principales: Sarmast, Mostafa Khoshhal, Salehi, Hassan, Khosh-Khui, Morteza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235888/
https://www.ncbi.nlm.nih.gov/pubmed/28324305
http://dx.doi.org/10.1007/s13205-013-0191-8
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author Sarmast, Mostafa Khoshhal
Salehi, Hassan
Khosh-Khui, Morteza
author_facet Sarmast, Mostafa Khoshhal
Salehi, Hassan
Khosh-Khui, Morteza
author_sort Sarmast, Mostafa Khoshhal
collection PubMed
description Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these plantlets is the most important step in tissue culture techniques. An experiment was conducted under in vitro conditions to study the effects of shaking duration (twice daily at 6:00 a.m. and 9:00 p.m. for 2, 4, 8, and 16 min at 250 rpm for 14 days) on Sansevieria trifasciata L. as a model plant. Results showed that shaking improved handling, total plant height, and leaf characteristics of the model plant. Forty-eight hours after 14 days of shaking treatments with increasing shaking time, leaf length decreased but proline content of leaf increased. However, 6 months after starting the experiment different results were observed. In explants that received 16 min of shaking treatment, leaf length and area and photosynthesis rate were increased compared with control plantlets. Six months after starting the experiment, control plantlets had 12.5 % mortality; however, no mortality was observed in other treated explants. The results demonstrated that shaking improved the explants’ root length and number and as a simple, cost-effective, and non-chemical novel approach may be substituted for other prevalent acclimatization techniques used for tissue culture regenerated plantlets. Further studies with sensitive plants are needed to establish this hypothesis.
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spelling pubmed-42358882014-11-20 Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants Sarmast, Mostafa Khoshhal Salehi, Hassan Khosh-Khui, Morteza 3 Biotech Original Article Plantlets under in vitro conditions transferred to ex vivo conditions are exposed to biotic and abiotic stresses. Furthermore, in vitro regenerated plants are typically frail and sometimes difficult to handle subsequently increasing their risk to damage and disease; hence acclimatization of these plantlets is the most important step in tissue culture techniques. An experiment was conducted under in vitro conditions to study the effects of shaking duration (twice daily at 6:00 a.m. and 9:00 p.m. for 2, 4, 8, and 16 min at 250 rpm for 14 days) on Sansevieria trifasciata L. as a model plant. Results showed that shaking improved handling, total plant height, and leaf characteristics of the model plant. Forty-eight hours after 14 days of shaking treatments with increasing shaking time, leaf length decreased but proline content of leaf increased. However, 6 months after starting the experiment different results were observed. In explants that received 16 min of shaking treatment, leaf length and area and photosynthesis rate were increased compared with control plantlets. Six months after starting the experiment, control plantlets had 12.5 % mortality; however, no mortality was observed in other treated explants. The results demonstrated that shaking improved the explants’ root length and number and as a simple, cost-effective, and non-chemical novel approach may be substituted for other prevalent acclimatization techniques used for tissue culture regenerated plantlets. Further studies with sensitive plants are needed to establish this hypothesis. Springer Berlin Heidelberg 2013-12-25 2014-12 /pmc/articles/PMC4235888/ /pubmed/28324305 http://dx.doi.org/10.1007/s13205-013-0191-8 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/This article is published under license to BioMed Central Ltd. Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Sarmast, Mostafa Khoshhal
Salehi, Hassan
Khosh-Khui, Morteza
Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants
title Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants
title_full Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants
title_fullStr Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants
title_full_unstemmed Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants
title_short Seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants
title_sort seismomorphogenesis: a novel approach to acclimatization of tissue culture regenerated plants
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235888/
https://www.ncbi.nlm.nih.gov/pubmed/28324305
http://dx.doi.org/10.1007/s13205-013-0191-8
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