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Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China
BACKGROUND: The rapid emergence and dissemination of carbapenem resistance in Enterobacteriaceae complicates the treatment of infections caused by these organisms. METHODS: We collected clinical isolates with meropenem inhibition zones of ≤ 22 mm from January 1, 2009, through December 31, 2010. We a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236511/ https://www.ncbi.nlm.nih.gov/pubmed/25113057 http://dx.doi.org/10.1186/s12941-014-0042-4 |
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author | Chen, Sheng Feng, Wei Chen, Jianhong Liao, Wei He, Nianhai Wang, Qian Sun, Fengjun Xia, Peiyuan |
author_facet | Chen, Sheng Feng, Wei Chen, Jianhong Liao, Wei He, Nianhai Wang, Qian Sun, Fengjun Xia, Peiyuan |
author_sort | Chen, Sheng |
collection | PubMed |
description | BACKGROUND: The rapid emergence and dissemination of carbapenem resistance in Enterobacteriaceae complicates the treatment of infections caused by these organisms. METHODS: We collected clinical isolates with meropenem inhibition zones of ≤ 22 mm from January 1, 2009, through December 31, 2010. We attempted to amplify the NDM-1 gene from these isolates and conducted the modified Hodge test (MHT). The minimal inhibitory concentration (MIC) of the MHT-positive strains was determined by the agar disk dilution method. The carbapenemase-encoding resistance genes of these strains were examined using polymerase chain reaction (PCR) analysis and a sequencing strategy to characterize these enzymes. The clonal relationship among isolates was analyzed by pulsed-field gel electrophoresis (PFGE). RESULTS: Among the 158 Enterobacteriaceae isolates that were collected, there were no NDM-1-positive strains and 26 MHT-positive strains. Among the latter, 18 strains were IMP-4-positive, and 1 was KPC-2-positive. In addition, 15 of the IMP-4-positive Klebsiella pneumoniae strains belonged to 4 PFGE genotypes, with 8 strains having the same genotype. CONCLUSION: These results suggest that nosocomial infections are one of the main reasons for the spread of these resistant strains. |
format | Online Article Text |
id | pubmed-4236511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42365112014-11-19 Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China Chen, Sheng Feng, Wei Chen, Jianhong Liao, Wei He, Nianhai Wang, Qian Sun, Fengjun Xia, Peiyuan Ann Clin Microbiol Antimicrob Research BACKGROUND: The rapid emergence and dissemination of carbapenem resistance in Enterobacteriaceae complicates the treatment of infections caused by these organisms. METHODS: We collected clinical isolates with meropenem inhibition zones of ≤ 22 mm from January 1, 2009, through December 31, 2010. We attempted to amplify the NDM-1 gene from these isolates and conducted the modified Hodge test (MHT). The minimal inhibitory concentration (MIC) of the MHT-positive strains was determined by the agar disk dilution method. The carbapenemase-encoding resistance genes of these strains were examined using polymerase chain reaction (PCR) analysis and a sequencing strategy to characterize these enzymes. The clonal relationship among isolates was analyzed by pulsed-field gel electrophoresis (PFGE). RESULTS: Among the 158 Enterobacteriaceae isolates that were collected, there were no NDM-1-positive strains and 26 MHT-positive strains. Among the latter, 18 strains were IMP-4-positive, and 1 was KPC-2-positive. In addition, 15 of the IMP-4-positive Klebsiella pneumoniae strains belonged to 4 PFGE genotypes, with 8 strains having the same genotype. CONCLUSION: These results suggest that nosocomial infections are one of the main reasons for the spread of these resistant strains. BioMed Central 2014-08-12 /pmc/articles/PMC4236511/ /pubmed/25113057 http://dx.doi.org/10.1186/s12941-014-0042-4 Text en Copyright © 2014 Chen et al.; licensee BioMed Central Ltd http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Chen, Sheng Feng, Wei Chen, Jianhong Liao, Wei He, Nianhai Wang, Qian Sun, Fengjun Xia, Peiyuan Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China |
title | Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China |
title_full | Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China |
title_fullStr | Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China |
title_full_unstemmed | Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China |
title_short | Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China |
title_sort | spread of carbapenemase-producing enterobacteria in a southwest hospital in china |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236511/ https://www.ncbi.nlm.nih.gov/pubmed/25113057 http://dx.doi.org/10.1186/s12941-014-0042-4 |
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