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Polymerase chain reaction–based assays for the diagnosis of human brucellosis
Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification, which is commonly used to diagnose infectious diseases. The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236518/ https://www.ncbi.nlm.nih.gov/pubmed/25082566 http://dx.doi.org/10.1186/s12941-014-0031-7 |
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author | Wang, Ying Wang, Zhanli Zhang, Yaxian Bai, Liyun Zhao, Yue Liu, Chunfang Ma, An Yu, Hui |
author_facet | Wang, Ying Wang, Zhanli Zhang, Yaxian Bai, Liyun Zhao, Yue Liu, Chunfang Ma, An Yu, Hui |
author_sort | Wang, Ying |
collection | PubMed |
description | Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification, which is commonly used to diagnose infectious diseases. The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility and technical ease. Brucellosis is a common zoonosis caused by Brucella spp., which still remains as a major health problem in many developing countries around the world. The direct culture and immunohistochemistry can be used for detecting infection with Brucella spp. However, PCR has the potential to address limitations of these methods. PCR are now one of the most useful assays for the diagnosis in human brucellosis. The aim of this review was to summarize the main PCR techniques and their applications for diagnosis and follow-up of patients with brucellosis. Moreover, advantages or limitation of the different PCR methods as well as the evaluation of PCR results for treatment and follow-up of human brucellosis were also discussed. |
format | Online Article Text |
id | pubmed-4236518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42365182014-11-19 Polymerase chain reaction–based assays for the diagnosis of human brucellosis Wang, Ying Wang, Zhanli Zhang, Yaxian Bai, Liyun Zhao, Yue Liu, Chunfang Ma, An Yu, Hui Ann Clin Microbiol Antimicrob Review Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification, which is commonly used to diagnose infectious diseases. The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility and technical ease. Brucellosis is a common zoonosis caused by Brucella spp., which still remains as a major health problem in many developing countries around the world. The direct culture and immunohistochemistry can be used for detecting infection with Brucella spp. However, PCR has the potential to address limitations of these methods. PCR are now one of the most useful assays for the diagnosis in human brucellosis. The aim of this review was to summarize the main PCR techniques and their applications for diagnosis and follow-up of patients with brucellosis. Moreover, advantages or limitation of the different PCR methods as well as the evaluation of PCR results for treatment and follow-up of human brucellosis were also discussed. BioMed Central 2014-08-01 /pmc/articles/PMC4236518/ /pubmed/25082566 http://dx.doi.org/10.1186/s12941-014-0031-7 Text en Copyright © 2014 Wang et al.; licensee Biomed Central Ltd http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Review Wang, Ying Wang, Zhanli Zhang, Yaxian Bai, Liyun Zhao, Yue Liu, Chunfang Ma, An Yu, Hui Polymerase chain reaction–based assays for the diagnosis of human brucellosis |
title | Polymerase chain reaction–based assays for the diagnosis of human brucellosis |
title_full | Polymerase chain reaction–based assays for the diagnosis of human brucellosis |
title_fullStr | Polymerase chain reaction–based assays for the diagnosis of human brucellosis |
title_full_unstemmed | Polymerase chain reaction–based assays for the diagnosis of human brucellosis |
title_short | Polymerase chain reaction–based assays for the diagnosis of human brucellosis |
title_sort | polymerase chain reaction–based assays for the diagnosis of human brucellosis |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236518/ https://www.ncbi.nlm.nih.gov/pubmed/25082566 http://dx.doi.org/10.1186/s12941-014-0031-7 |
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