Cargando…

Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate

BACKGROUND: Polyphosphate (polyP) has bactericidal activity against a gram-negative periodontopathogen Porphyromonas gingivalis, a black-pigmented gram-negative anaerobic rod. However, current knowledge about the mode of action of polyP against P. gingivalis is incomplete. To elucidate the mechanism...

Descripción completa

Detalles Bibliográficos
Autores principales: Moon, Ji-Hoi, Lee, Jae-Hyung, Lee, Jin-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236598/
https://www.ncbi.nlm.nih.gov/pubmed/25148905
http://dx.doi.org/10.1186/s12866-014-0218-2
_version_ 1782345198766915584
author Moon, Ji-Hoi
Lee, Jae-Hyung
Lee, Jin-Yong
author_facet Moon, Ji-Hoi
Lee, Jae-Hyung
Lee, Jin-Yong
author_sort Moon, Ji-Hoi
collection PubMed
description BACKGROUND: Polyphosphate (polyP) has bactericidal activity against a gram-negative periodontopathogen Porphyromonas gingivalis, a black-pigmented gram-negative anaerobic rod. However, current knowledge about the mode of action of polyP against P. gingivalis is incomplete. To elucidate the mechanisms of antibacterial action of polyP against P. gingivalis, we performed the full-genome gene expression microarrays, and gene ontology (GO) and protein-protein interaction network analysis of differentially expressed genes (DEGs). RESULTS: We successfully identified 349 up-regulated genes and 357 down-regulated genes (>1.5-fold, P < 0.05) in P. gingivalis W83 treated with polyP75 (sodium polyphosphate, Na(n+2)P(n)O(3n+1); n = 75). Real-time PCR confirmed the up- and down-regulation of some selected genes. GO analysis of the DEGs identified distinct biological themes. Using 202 DEGs belonging to the biological themes, we generated the protein-protein interaction network based on a database of known and predicted protein interactions. The network analysis identified biological meaningful clusters related to hemin acquisition, energy metabolism, cell envelope and cell division, ribosomal proteins, and transposon function. CONCLUSIONS: polyP probably exerts its antibacterial effect through inhibition of hemin acquisition by the bacterium, resulting in severe perturbation of energy metabolism, cell envelope biosynthesis and cell division, and elevated transposition. Further studies will be needed to elucidate the exact mechanism by which polyP induces up-regulation of the genes related to ribosomal proteins. Our results will shed new light on the study of the antibacterial mechanism of polyP against other related bacteria belonging to the black-pigmented Bacteroides species.
format Online
Article
Text
id pubmed-4236598
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-42365982014-11-19 Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate Moon, Ji-Hoi Lee, Jae-Hyung Lee, Jin-Yong BMC Microbiol Research Article BACKGROUND: Polyphosphate (polyP) has bactericidal activity against a gram-negative periodontopathogen Porphyromonas gingivalis, a black-pigmented gram-negative anaerobic rod. However, current knowledge about the mode of action of polyP against P. gingivalis is incomplete. To elucidate the mechanisms of antibacterial action of polyP against P. gingivalis, we performed the full-genome gene expression microarrays, and gene ontology (GO) and protein-protein interaction network analysis of differentially expressed genes (DEGs). RESULTS: We successfully identified 349 up-regulated genes and 357 down-regulated genes (>1.5-fold, P < 0.05) in P. gingivalis W83 treated with polyP75 (sodium polyphosphate, Na(n+2)P(n)O(3n+1); n = 75). Real-time PCR confirmed the up- and down-regulation of some selected genes. GO analysis of the DEGs identified distinct biological themes. Using 202 DEGs belonging to the biological themes, we generated the protein-protein interaction network based on a database of known and predicted protein interactions. The network analysis identified biological meaningful clusters related to hemin acquisition, energy metabolism, cell envelope and cell division, ribosomal proteins, and transposon function. CONCLUSIONS: polyP probably exerts its antibacterial effect through inhibition of hemin acquisition by the bacterium, resulting in severe perturbation of energy metabolism, cell envelope biosynthesis and cell division, and elevated transposition. Further studies will be needed to elucidate the exact mechanism by which polyP induces up-regulation of the genes related to ribosomal proteins. Our results will shed new light on the study of the antibacterial mechanism of polyP against other related bacteria belonging to the black-pigmented Bacteroides species. BioMed Central 2014-08-24 /pmc/articles/PMC4236598/ /pubmed/25148905 http://dx.doi.org/10.1186/s12866-014-0218-2 Text en Copyright © 2014 Moon et al.; licensee BioMed Central Ltd http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Moon, Ji-Hoi
Lee, Jae-Hyung
Lee, Jin-Yong
Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
title Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
title_full Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
title_fullStr Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
title_full_unstemmed Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
title_short Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate
title_sort microarray analysis of the transcriptional responses of porphyromonas gingivalis to polyphosphate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236598/
https://www.ncbi.nlm.nih.gov/pubmed/25148905
http://dx.doi.org/10.1186/s12866-014-0218-2
work_keys_str_mv AT moonjihoi microarrayanalysisofthetranscriptionalresponsesofporphyromonasgingivalistopolyphosphate
AT leejaehyung microarrayanalysisofthetranscriptionalresponsesofporphyromonasgingivalistopolyphosphate
AT leejinyong microarrayanalysisofthetranscriptionalresponsesofporphyromonasgingivalistopolyphosphate