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Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors
Hepatitis C virus (HCV) replication is dependent on a liver-specific microRNA (miRNA), miR-122. A recent clinical trial reported that transient inhibition of miR-122 reduced viral titers in HCV infected patients. Here we set out to better understand how miR-122 inhibition influences HCV replication...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236719/ https://www.ncbi.nlm.nih.gov/pubmed/25403145 http://dx.doi.org/10.1038/ncomms6408 |
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author | Israelow, Benjamin Mullokandov, Gavriel Agudo, Judith Sourisseau, Marion Bashir, Ali Maldonado, Andres Y. Dar, Arvin C. Brown, Brian D. Evans, Matthew J. |
author_facet | Israelow, Benjamin Mullokandov, Gavriel Agudo, Judith Sourisseau, Marion Bashir, Ali Maldonado, Andres Y. Dar, Arvin C. Brown, Brian D. Evans, Matthew J. |
author_sort | Israelow, Benjamin |
collection | PubMed |
description | Hepatitis C virus (HCV) replication is dependent on a liver-specific microRNA (miRNA), miR-122. A recent clinical trial reported that transient inhibition of miR-122 reduced viral titers in HCV infected patients. Here we set out to better understand how miR-122 inhibition influences HCV replication over time. Unexpectedly, we observed the emergence of a HCV variant that is resistant to miR-122 knockdown. Next-generation sequencing revealed that this was due to a single nucleotide change at position 28 (G28A) of the HCV genome, which falls between the two miR-122 seed-binding sites. Naturally occurring HCV isolates encoding G28A are similarly resistant to miR-122 inhibition, indicating that subtle differences in viral sequence, even outside the seed-binding site, greatly influence HCV’s miR-122 concentration requirement. Additionally, we found that HCV itself reduces miR-122’s activity in the cell, possibly through binding and sequestering miR-122. Our study provides insight into the interaction between miR-122 and HCV, including viral adaptation to reduced miR-122 bioavailability, and has implications for the development of anti-miR-122-based HCV drugs. |
format | Online Article Text |
id | pubmed-4236719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-42367192015-05-18 Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors Israelow, Benjamin Mullokandov, Gavriel Agudo, Judith Sourisseau, Marion Bashir, Ali Maldonado, Andres Y. Dar, Arvin C. Brown, Brian D. Evans, Matthew J. Nat Commun Article Hepatitis C virus (HCV) replication is dependent on a liver-specific microRNA (miRNA), miR-122. A recent clinical trial reported that transient inhibition of miR-122 reduced viral titers in HCV infected patients. Here we set out to better understand how miR-122 inhibition influences HCV replication over time. Unexpectedly, we observed the emergence of a HCV variant that is resistant to miR-122 knockdown. Next-generation sequencing revealed that this was due to a single nucleotide change at position 28 (G28A) of the HCV genome, which falls between the two miR-122 seed-binding sites. Naturally occurring HCV isolates encoding G28A are similarly resistant to miR-122 inhibition, indicating that subtle differences in viral sequence, even outside the seed-binding site, greatly influence HCV’s miR-122 concentration requirement. Additionally, we found that HCV itself reduces miR-122’s activity in the cell, possibly through binding and sequestering miR-122. Our study provides insight into the interaction between miR-122 and HCV, including viral adaptation to reduced miR-122 bioavailability, and has implications for the development of anti-miR-122-based HCV drugs. 2014-11-18 /pmc/articles/PMC4236719/ /pubmed/25403145 http://dx.doi.org/10.1038/ncomms6408 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Israelow, Benjamin Mullokandov, Gavriel Agudo, Judith Sourisseau, Marion Bashir, Ali Maldonado, Andres Y. Dar, Arvin C. Brown, Brian D. Evans, Matthew J. Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors |
title | Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors |
title_full | Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors |
title_fullStr | Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors |
title_full_unstemmed | Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors |
title_short | Hepatitis C virus genetics affects miR-122 requirements and response to miR-122 inhibitors |
title_sort | hepatitis c virus genetics affects mir-122 requirements and response to mir-122 inhibitors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236719/ https://www.ncbi.nlm.nih.gov/pubmed/25403145 http://dx.doi.org/10.1038/ncomms6408 |
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