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Love makes smell blind: mating suppresses pheromone attraction in Drosophila females via Or65a olfactory neurons

In Drosophila, the male sex pheromone cis-vaccenyl acetate (cVA) elicits aggregation and courtship, through the odorant receptor Or67d. Long-lasting exposure to cVA suppresses male courtship, via a second channel, Or65a. In females, the role of Or65a has not been studied. We show that, shortly after...

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Autores principales: Lebreton, Sébastien, Grabe, Veit, Omondi, Aman B., Ignell, Rickard, Becher, Paul G., Hansson, Bill S., Sachse, Silke, Witzgall, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236738/
https://www.ncbi.nlm.nih.gov/pubmed/25406576
http://dx.doi.org/10.1038/srep07119
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author Lebreton, Sébastien
Grabe, Veit
Omondi, Aman B.
Ignell, Rickard
Becher, Paul G.
Hansson, Bill S.
Sachse, Silke
Witzgall, Peter
author_facet Lebreton, Sébastien
Grabe, Veit
Omondi, Aman B.
Ignell, Rickard
Becher, Paul G.
Hansson, Bill S.
Sachse, Silke
Witzgall, Peter
author_sort Lebreton, Sébastien
collection PubMed
description In Drosophila, the male sex pheromone cis-vaccenyl acetate (cVA) elicits aggregation and courtship, through the odorant receptor Or67d. Long-lasting exposure to cVA suppresses male courtship, via a second channel, Or65a. In females, the role of Or65a has not been studied. We show that, shortly after mating, Drosophila females are no longer attracted to cVA and that activation of olfactory sensory neurons (OSNs) expressing Or65a generates this behavioral switch: when silencing Or65a, mated females remain responsive to cVA. Neurons expressing Or67d converge into the DA1 glomerulus in the antennal lobe, where they synapse onto projection neurons (PNs), that connect to higher neural circuits generating the attraction response to cVA. Functional imaging of these PNs shows that the DA1 glomerulus is inhibited by simultaneous activation of Or65a OSNs, which leads to a suppression of the attraction response to cVA. The behavioral role of postmating cVA exposure is substantiated by the observation that matings with starved males, which produce less cVA, do not alter the female response. Moreover, exposure to synthetic cVA abolishes attraction and decreases sexual receptivity in unmated females. Taken together, Or65a mediates an aversive effect of cVA and may accordingly regulate remating, through concurrent behavioral modulation in males and females.
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spelling pubmed-42367382014-11-25 Love makes smell blind: mating suppresses pheromone attraction in Drosophila females via Or65a olfactory neurons Lebreton, Sébastien Grabe, Veit Omondi, Aman B. Ignell, Rickard Becher, Paul G. Hansson, Bill S. Sachse, Silke Witzgall, Peter Sci Rep Article In Drosophila, the male sex pheromone cis-vaccenyl acetate (cVA) elicits aggregation and courtship, through the odorant receptor Or67d. Long-lasting exposure to cVA suppresses male courtship, via a second channel, Or65a. In females, the role of Or65a has not been studied. We show that, shortly after mating, Drosophila females are no longer attracted to cVA and that activation of olfactory sensory neurons (OSNs) expressing Or65a generates this behavioral switch: when silencing Or65a, mated females remain responsive to cVA. Neurons expressing Or67d converge into the DA1 glomerulus in the antennal lobe, where they synapse onto projection neurons (PNs), that connect to higher neural circuits generating the attraction response to cVA. Functional imaging of these PNs shows that the DA1 glomerulus is inhibited by simultaneous activation of Or65a OSNs, which leads to a suppression of the attraction response to cVA. The behavioral role of postmating cVA exposure is substantiated by the observation that matings with starved males, which produce less cVA, do not alter the female response. Moreover, exposure to synthetic cVA abolishes attraction and decreases sexual receptivity in unmated females. Taken together, Or65a mediates an aversive effect of cVA and may accordingly regulate remating, through concurrent behavioral modulation in males and females. Nature Publishing Group 2014-11-19 /pmc/articles/PMC4236738/ /pubmed/25406576 http://dx.doi.org/10.1038/srep07119 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Lebreton, Sébastien
Grabe, Veit
Omondi, Aman B.
Ignell, Rickard
Becher, Paul G.
Hansson, Bill S.
Sachse, Silke
Witzgall, Peter
Love makes smell blind: mating suppresses pheromone attraction in Drosophila females via Or65a olfactory neurons
title Love makes smell blind: mating suppresses pheromone attraction in Drosophila females via Or65a olfactory neurons
title_full Love makes smell blind: mating suppresses pheromone attraction in Drosophila females via Or65a olfactory neurons
title_fullStr Love makes smell blind: mating suppresses pheromone attraction in Drosophila females via Or65a olfactory neurons
title_full_unstemmed Love makes smell blind: mating suppresses pheromone attraction in Drosophila females via Or65a olfactory neurons
title_short Love makes smell blind: mating suppresses pheromone attraction in Drosophila females via Or65a olfactory neurons
title_sort love makes smell blind: mating suppresses pheromone attraction in drosophila females via or65a olfactory neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236738/
https://www.ncbi.nlm.nih.gov/pubmed/25406576
http://dx.doi.org/10.1038/srep07119
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