Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3

The centromere-specific histone H3 variant, CENP-A, is overexpressed in particular aggressive cancer cells, where it can be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3. In the present study, we report the crystal structure of the heterotypic CENP-A/H3.3 particle a...

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Autores principales: Arimura, Yasuhiro, Shirayama, Kazuyoshi, Horikoshi, Naoki, Fujita, Risa, Taguchi, Hiroyuki, Kagawa, Wataru, Fukagawa, Tatsuo, Almouzni, Geneviève, Kurumizaka, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236741/
https://www.ncbi.nlm.nih.gov/pubmed/25408271
http://dx.doi.org/10.1038/srep07115
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author Arimura, Yasuhiro
Shirayama, Kazuyoshi
Horikoshi, Naoki
Fujita, Risa
Taguchi, Hiroyuki
Kagawa, Wataru
Fukagawa, Tatsuo
Almouzni, Geneviève
Kurumizaka, Hitoshi
author_facet Arimura, Yasuhiro
Shirayama, Kazuyoshi
Horikoshi, Naoki
Fujita, Risa
Taguchi, Hiroyuki
Kagawa, Wataru
Fukagawa, Tatsuo
Almouzni, Geneviève
Kurumizaka, Hitoshi
author_sort Arimura, Yasuhiro
collection PubMed
description The centromere-specific histone H3 variant, CENP-A, is overexpressed in particular aggressive cancer cells, where it can be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3. In the present study, we report the crystal structure of the heterotypic CENP-A/H3.3 particle and reveal its “hybrid structure”, in which the physical characteristics of CENP-A and H3.3 are conserved independently within the same particle. The CENP-A/H3.3 nucleosome forms an unexpectedly stable structure as compared to the CENP-A nucleosome, and allows the binding of the essential centromeric protein, CENP-C, which is ectopically mislocalized in the chromosomes of CENP-A overexpressing cells.
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spelling pubmed-42367412014-11-25 Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3 Arimura, Yasuhiro Shirayama, Kazuyoshi Horikoshi, Naoki Fujita, Risa Taguchi, Hiroyuki Kagawa, Wataru Fukagawa, Tatsuo Almouzni, Geneviève Kurumizaka, Hitoshi Sci Rep Article The centromere-specific histone H3 variant, CENP-A, is overexpressed in particular aggressive cancer cells, where it can be mislocalized ectopically in the form of heterotypic nucleosomes containing H3.3. In the present study, we report the crystal structure of the heterotypic CENP-A/H3.3 particle and reveal its “hybrid structure”, in which the physical characteristics of CENP-A and H3.3 are conserved independently within the same particle. The CENP-A/H3.3 nucleosome forms an unexpectedly stable structure as compared to the CENP-A nucleosome, and allows the binding of the essential centromeric protein, CENP-C, which is ectopically mislocalized in the chromosomes of CENP-A overexpressing cells. Nature Publishing Group 2014-11-19 /pmc/articles/PMC4236741/ /pubmed/25408271 http://dx.doi.org/10.1038/srep07115 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Arimura, Yasuhiro
Shirayama, Kazuyoshi
Horikoshi, Naoki
Fujita, Risa
Taguchi, Hiroyuki
Kagawa, Wataru
Fukagawa, Tatsuo
Almouzni, Geneviève
Kurumizaka, Hitoshi
Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
title Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
title_full Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
title_fullStr Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
title_full_unstemmed Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
title_short Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3
title_sort crystal structure and stable property of the cancer-associated heterotypic nucleosome containing cenp-a and h3.3
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236741/
https://www.ncbi.nlm.nih.gov/pubmed/25408271
http://dx.doi.org/10.1038/srep07115
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