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Changes in the cellular proteins of A549 infected with Hepatitis E virus by proteomics analysis
BACKGROUND: Our understanding of Hepatitis E virus (HEV) has changed enormously over the past 30 years, from a waterborne infection causing outbreaks of acute hepatitis in developing countries to an infection of global distribution causing a range of hepatic and extra-hepatic illness. However, the k...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236826/ https://www.ncbi.nlm.nih.gov/pubmed/25175408 http://dx.doi.org/10.1186/s12917-014-0188-5 |
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author | Shen, Quan Pu, Yingyan Fu, Xingli Xie, Ying Bian, Xiaobo Yang, Shixing Yang, Yan Cui, Li Wang, Xiaochun Wang, Hua Zhang, Wen |
author_facet | Shen, Quan Pu, Yingyan Fu, Xingli Xie, Ying Bian, Xiaobo Yang, Shixing Yang, Yan Cui, Li Wang, Xiaochun Wang, Hua Zhang, Wen |
author_sort | Shen, Quan |
collection | PubMed |
description | BACKGROUND: Our understanding of Hepatitis E virus (HEV) has changed enormously over the past 30 years, from a waterborne infection causing outbreaks of acute hepatitis in developing countries to an infection of global distribution causing a range of hepatic and extra-hepatic illness. However, the key proteins playing important parts in the virus infection were still unknown. Understanding the changes of cellular proteins in these cells exposed to HEV is helpful for elucidating molecular mechanisms associated with function alterations of HEV-infected susceptible cells. In the present study, a comparative gel-based proteomic analysis was employed to study the changes in cellular proteins of A549 exposed to HEV in vitro to provide novel information for understanding the functional alterations of A549 induced by HEV infection. RESULT: Of 2 585-3 152 protein spots visualized on each gel using silver staining, a total of 31 protein spots were found to be differentially expressed in HEV-infected A549 cells compared with mock-infected A549, including 10 significantly up-regulated protein spots and 21 significantly down-regulated protein spots. CONCLUSION: Our work is the first time regarding the proteomic analysis on the cellular responses to HEV infection. This work is helpful for investigating the molecular basis associated with the interaction between HEV and the host cells although more efforts should be required to discover the mechanisms. |
format | Online Article Text |
id | pubmed-4236826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42368262014-11-20 Changes in the cellular proteins of A549 infected with Hepatitis E virus by proteomics analysis Shen, Quan Pu, Yingyan Fu, Xingli Xie, Ying Bian, Xiaobo Yang, Shixing Yang, Yan Cui, Li Wang, Xiaochun Wang, Hua Zhang, Wen BMC Vet Res Research Article BACKGROUND: Our understanding of Hepatitis E virus (HEV) has changed enormously over the past 30 years, from a waterborne infection causing outbreaks of acute hepatitis in developing countries to an infection of global distribution causing a range of hepatic and extra-hepatic illness. However, the key proteins playing important parts in the virus infection were still unknown. Understanding the changes of cellular proteins in these cells exposed to HEV is helpful for elucidating molecular mechanisms associated with function alterations of HEV-infected susceptible cells. In the present study, a comparative gel-based proteomic analysis was employed to study the changes in cellular proteins of A549 exposed to HEV in vitro to provide novel information for understanding the functional alterations of A549 induced by HEV infection. RESULT: Of 2 585-3 152 protein spots visualized on each gel using silver staining, a total of 31 protein spots were found to be differentially expressed in HEV-infected A549 cells compared with mock-infected A549, including 10 significantly up-regulated protein spots and 21 significantly down-regulated protein spots. CONCLUSION: Our work is the first time regarding the proteomic analysis on the cellular responses to HEV infection. This work is helpful for investigating the molecular basis associated with the interaction between HEV and the host cells although more efforts should be required to discover the mechanisms. BioMed Central 2014-08-30 /pmc/articles/PMC4236826/ /pubmed/25175408 http://dx.doi.org/10.1186/s12917-014-0188-5 Text en Copyright © 2014 Shen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Shen, Quan Pu, Yingyan Fu, Xingli Xie, Ying Bian, Xiaobo Yang, Shixing Yang, Yan Cui, Li Wang, Xiaochun Wang, Hua Zhang, Wen Changes in the cellular proteins of A549 infected with Hepatitis E virus by proteomics analysis |
title | Changes in the cellular proteins of A549 infected with Hepatitis E virus by proteomics analysis |
title_full | Changes in the cellular proteins of A549 infected with Hepatitis E virus by proteomics analysis |
title_fullStr | Changes in the cellular proteins of A549 infected with Hepatitis E virus by proteomics analysis |
title_full_unstemmed | Changes in the cellular proteins of A549 infected with Hepatitis E virus by proteomics analysis |
title_short | Changes in the cellular proteins of A549 infected with Hepatitis E virus by proteomics analysis |
title_sort | changes in the cellular proteins of a549 infected with hepatitis e virus by proteomics analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236826/ https://www.ncbi.nlm.nih.gov/pubmed/25175408 http://dx.doi.org/10.1186/s12917-014-0188-5 |
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