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Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses
BACKGROUND: Phenotypic plasticity in defensive traits occurs in many species when facing heterogeneous predator regimes. The waterflea Daphnia is well-known for showing a variety of these so called inducible defences. However, molecular mechanisms underlying this plasticity are poorly understood so...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236883/ https://www.ncbi.nlm.nih.gov/pubmed/24762235 http://dx.doi.org/10.1186/1471-2164-15-306 |
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author | Otte, Kathrin A Fröhlich, Thomas Arnold, Georg J Laforsch, Christian |
author_facet | Otte, Kathrin A Fröhlich, Thomas Arnold, Georg J Laforsch, Christian |
author_sort | Otte, Kathrin A |
collection | PubMed |
description | BACKGROUND: Phenotypic plasticity in defensive traits occurs in many species when facing heterogeneous predator regimes. The waterflea Daphnia is well-known for showing a variety of these so called inducible defences. However, molecular mechanisms underlying this plasticity are poorly understood so far. We performed proteomic analysis on Daphnia magna exposed to chemical cues of the predator Triops cancriformis. D. magna develops an array of morphological changes in the presence of Triops including changes of carapace morphology and cuticle hardening. RESULTS: Using the 2D-DIGE technique, 1500 protein spots could be matched and quantified. We discovered 179 protein spots with altered intensity when comparing Triops exposed animals to a control group, and 69 spots were identified using nano-LC MS/MS. Kairomone exposure increased the intensity of spots containing muscle proteins, cuticle proteins and chitin-modifying enzymes as well as enzymes of carbohydrate and energy metabolism. The yolk precursor protein vitellogenin decreased in abundance in 41 of 43 spots. CONCLUSION: Identified proteins may be either directly involved in carapace stability or reflect changes in energy demand and allocation costs in animals exposed to predator kairomones. Our results present promising candidate proteins involved in the expression of inducible defences in Daphnia and enable further in depth analysis of this phenomenon. |
format | Online Article Text |
id | pubmed-4236883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42368832014-11-20 Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses Otte, Kathrin A Fröhlich, Thomas Arnold, Georg J Laforsch, Christian BMC Genomics Research Article BACKGROUND: Phenotypic plasticity in defensive traits occurs in many species when facing heterogeneous predator regimes. The waterflea Daphnia is well-known for showing a variety of these so called inducible defences. However, molecular mechanisms underlying this plasticity are poorly understood so far. We performed proteomic analysis on Daphnia magna exposed to chemical cues of the predator Triops cancriformis. D. magna develops an array of morphological changes in the presence of Triops including changes of carapace morphology and cuticle hardening. RESULTS: Using the 2D-DIGE technique, 1500 protein spots could be matched and quantified. We discovered 179 protein spots with altered intensity when comparing Triops exposed animals to a control group, and 69 spots were identified using nano-LC MS/MS. Kairomone exposure increased the intensity of spots containing muscle proteins, cuticle proteins and chitin-modifying enzymes as well as enzymes of carbohydrate and energy metabolism. The yolk precursor protein vitellogenin decreased in abundance in 41 of 43 spots. CONCLUSION: Identified proteins may be either directly involved in carapace stability or reflect changes in energy demand and allocation costs in animals exposed to predator kairomones. Our results present promising candidate proteins involved in the expression of inducible defences in Daphnia and enable further in depth analysis of this phenomenon. BioMed Central 2014-04-24 /pmc/articles/PMC4236883/ /pubmed/24762235 http://dx.doi.org/10.1186/1471-2164-15-306 Text en Copyright © 2014 Otte et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Otte, Kathrin A Fröhlich, Thomas Arnold, Georg J Laforsch, Christian Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses |
title | Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses |
title_full | Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses |
title_fullStr | Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses |
title_full_unstemmed | Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses |
title_short | Proteomic analysis of Daphnia magna hints at molecular pathways involved in defensive plastic responses |
title_sort | proteomic analysis of daphnia magna hints at molecular pathways involved in defensive plastic responses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236883/ https://www.ncbi.nlm.nih.gov/pubmed/24762235 http://dx.doi.org/10.1186/1471-2164-15-306 |
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