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IL-15 is decreased upon CsA and FK506 treatment of acute rejection following heart transplantation in mice
The aim of this study was to investigate the effect of cyclosporine A (CsA) and tacrolimus (FK506) on interleukin-15 (IL-15) production during acute rejection following heart transplantation in mice. Inbred male Balb/c (H-2d) and C57BL/6 (H-2b) mice were used to establish a heterotopic intra-abdomin...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237076/ https://www.ncbi.nlm.nih.gov/pubmed/25333459 http://dx.doi.org/10.3892/mmr.2014.2703 |
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author | YU, ZHIYONG ZHOU, XIAOPING YU, SONGFENG XIE, HAIYANG ZHENG, SHUSEN |
author_facet | YU, ZHIYONG ZHOU, XIAOPING YU, SONGFENG XIE, HAIYANG ZHENG, SHUSEN |
author_sort | YU, ZHIYONG |
collection | PubMed |
description | The aim of this study was to investigate the effect of cyclosporine A (CsA) and tacrolimus (FK506) on interleukin-15 (IL-15) production during acute rejection following heart transplantation in mice. Inbred male Balb/c (H-2d) and C57BL/6 (H-2b) mice were used to establish a heterotopic intra-abdominal cardiac transplantation model. The mice were divided in four groups: syngeneic control, allogeneic acute rejection, allogeneic rejection treated with CsA, and allogeneic rejection treated with FK506. The expression of IL-15, IL-2, and tumor necrosis factor-α (TNF-α) was measured using reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. A low level of IL-15 was detected in transplanted hearts of the control group, with a significant increase observed in the allogeneic acute rejection group. Compared to the allogeneic acute rejection group, IL-15 expression was significantly decreased in the CsA-and FK506-treated allogeneic rejection groups. The TNF-α expression pattern was similar to that of IL-15 in all groups. IL-2 expression was increased in the allogeneic acute rejection group and was inhibited in mice treated with CsA and FK506. In conclusion, increased IL-15 expression in rejected murine heart grafts may be reduced by CsA and FK506 in vivo. |
format | Online Article Text |
id | pubmed-4237076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-42370762014-11-19 IL-15 is decreased upon CsA and FK506 treatment of acute rejection following heart transplantation in mice YU, ZHIYONG ZHOU, XIAOPING YU, SONGFENG XIE, HAIYANG ZHENG, SHUSEN Mol Med Rep Articles The aim of this study was to investigate the effect of cyclosporine A (CsA) and tacrolimus (FK506) on interleukin-15 (IL-15) production during acute rejection following heart transplantation in mice. Inbred male Balb/c (H-2d) and C57BL/6 (H-2b) mice were used to establish a heterotopic intra-abdominal cardiac transplantation model. The mice were divided in four groups: syngeneic control, allogeneic acute rejection, allogeneic rejection treated with CsA, and allogeneic rejection treated with FK506. The expression of IL-15, IL-2, and tumor necrosis factor-α (TNF-α) was measured using reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. A low level of IL-15 was detected in transplanted hearts of the control group, with a significant increase observed in the allogeneic acute rejection group. Compared to the allogeneic acute rejection group, IL-15 expression was significantly decreased in the CsA-and FK506-treated allogeneic rejection groups. The TNF-α expression pattern was similar to that of IL-15 in all groups. IL-2 expression was increased in the allogeneic acute rejection group and was inhibited in mice treated with CsA and FK506. In conclusion, increased IL-15 expression in rejected murine heart grafts may be reduced by CsA and FK506 in vivo. D.A. Spandidos 2015-01 2014-10-20 /pmc/articles/PMC4237076/ /pubmed/25333459 http://dx.doi.org/10.3892/mmr.2014.2703 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles YU, ZHIYONG ZHOU, XIAOPING YU, SONGFENG XIE, HAIYANG ZHENG, SHUSEN IL-15 is decreased upon CsA and FK506 treatment of acute rejection following heart transplantation in mice |
title | IL-15 is decreased upon CsA and FK506 treatment of acute rejection following heart transplantation in mice |
title_full | IL-15 is decreased upon CsA and FK506 treatment of acute rejection following heart transplantation in mice |
title_fullStr | IL-15 is decreased upon CsA and FK506 treatment of acute rejection following heart transplantation in mice |
title_full_unstemmed | IL-15 is decreased upon CsA and FK506 treatment of acute rejection following heart transplantation in mice |
title_short | IL-15 is decreased upon CsA and FK506 treatment of acute rejection following heart transplantation in mice |
title_sort | il-15 is decreased upon csa and fk506 treatment of acute rejection following heart transplantation in mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237076/ https://www.ncbi.nlm.nih.gov/pubmed/25333459 http://dx.doi.org/10.3892/mmr.2014.2703 |
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