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Protein-protein interaction network and mechanism analysis in ischemic stroke
Ischemic stroke is a leading cause of mortality and permanent disability, with enormous financial repercussions on health systems worldwide. Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time. In order to examine the molecular mechanisms underlyi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237100/ https://www.ncbi.nlm.nih.gov/pubmed/25333814 http://dx.doi.org/10.3892/mmr.2014.2696 |
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author | QUAN, ZHE QUAN, YUAN WEI, BO FANG, DENING YU, WEIDONG JIA, HAO QUAN, WEI LIU, YUGUANG WANG, QIHONG |
author_facet | QUAN, ZHE QUAN, YUAN WEI, BO FANG, DENING YU, WEIDONG JIA, HAO QUAN, WEI LIU, YUGUANG WANG, QIHONG |
author_sort | QUAN, ZHE |
collection | PubMed |
description | Ischemic stroke is a leading cause of mortality and permanent disability, with enormous financial repercussions on health systems worldwide. Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time. In order to examine the molecular mechanisms underlying middle cerebral artery occlusion (MCAO)-induced ischemic stroke, the GSE35338 affymetrix microarray data was obtained from the Gene Expression Omnibus database and the differentially expressed genes (DEGs) between samples from patients with MCAO-induced ischemic stroke and sham controls at various time points were identified. Furthermore, protein-protein interaction (PPI) networks were constructed by mapping the DEGs into PPI data to identify the pathways that these DEGS are involved in. The results revealed that the expression of 438 DEGs, which are mainly involved in cell death, oxidant reduction, cell cycle and cell-cell signaling, were altered in MCAO samples. The nodes of CXC motif chemokine 10 (CXCL10) and interleukin-6 (IL-6) were large, with degrees of >20. In conclusion, the results suggest that CXCL10 and IL-6 have important roles in the occurrence and progression of MCAO-induced ischemic stroke. |
format | Online Article Text |
id | pubmed-4237100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-42371002014-11-19 Protein-protein interaction network and mechanism analysis in ischemic stroke QUAN, ZHE QUAN, YUAN WEI, BO FANG, DENING YU, WEIDONG JIA, HAO QUAN, WEI LIU, YUGUANG WANG, QIHONG Mol Med Rep Articles Ischemic stroke is a leading cause of mortality and permanent disability, with enormous financial repercussions on health systems worldwide. Ischemic brain injury results from a complex sequence of pathophysiological events that evolve over time. In order to examine the molecular mechanisms underlying middle cerebral artery occlusion (MCAO)-induced ischemic stroke, the GSE35338 affymetrix microarray data was obtained from the Gene Expression Omnibus database and the differentially expressed genes (DEGs) between samples from patients with MCAO-induced ischemic stroke and sham controls at various time points were identified. Furthermore, protein-protein interaction (PPI) networks were constructed by mapping the DEGs into PPI data to identify the pathways that these DEGS are involved in. The results revealed that the expression of 438 DEGs, which are mainly involved in cell death, oxidant reduction, cell cycle and cell-cell signaling, were altered in MCAO samples. The nodes of CXC motif chemokine 10 (CXCL10) and interleukin-6 (IL-6) were large, with degrees of >20. In conclusion, the results suggest that CXCL10 and IL-6 have important roles in the occurrence and progression of MCAO-induced ischemic stroke. D.A. Spandidos 2015-01 2014-10-17 /pmc/articles/PMC4237100/ /pubmed/25333814 http://dx.doi.org/10.3892/mmr.2014.2696 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles QUAN, ZHE QUAN, YUAN WEI, BO FANG, DENING YU, WEIDONG JIA, HAO QUAN, WEI LIU, YUGUANG WANG, QIHONG Protein-protein interaction network and mechanism analysis in ischemic stroke |
title | Protein-protein interaction network and mechanism analysis in ischemic stroke |
title_full | Protein-protein interaction network and mechanism analysis in ischemic stroke |
title_fullStr | Protein-protein interaction network and mechanism analysis in ischemic stroke |
title_full_unstemmed | Protein-protein interaction network and mechanism analysis in ischemic stroke |
title_short | Protein-protein interaction network and mechanism analysis in ischemic stroke |
title_sort | protein-protein interaction network and mechanism analysis in ischemic stroke |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237100/ https://www.ncbi.nlm.nih.gov/pubmed/25333814 http://dx.doi.org/10.3892/mmr.2014.2696 |
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