The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation

We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or resolvases. This method involves two successi...

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Detalles Bibliográficos
Autores principales: Bossé, Janine T., Soares-Bazzolli, Denise M., Li, Yanwen, Wren, Brendan W., Tucker, Alexander W., Maskell, Duncan J., Rycroft, Andrew N., Langford, Paul R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237320/
https://www.ncbi.nlm.nih.gov/pubmed/25409017
http://dx.doi.org/10.1371/journal.pone.0111252

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