Cargando…

Roles of Intragenic and Intergenic L1s in Mouse and Human

Long INterspersed Element-1 (LINE-1 or L1) is a retrotransposable element that has shaped the evolution of mammalian genomes. There is increasing evidence that transcriptionally active L1 could have been co-opted through evolution to play various roles including X-inactivation, homologous recombinat...

Descripción completa

Detalles Bibliográficos
Autores principales: Ngamphiw, Chumpol, Tongsima, Sissades, Mutirangura, Apiwat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237456/
https://www.ncbi.nlm.nih.gov/pubmed/25409429
http://dx.doi.org/10.1371/journal.pone.0113434
_version_ 1782345352784904192
author Ngamphiw, Chumpol
Tongsima, Sissades
Mutirangura, Apiwat
author_facet Ngamphiw, Chumpol
Tongsima, Sissades
Mutirangura, Apiwat
author_sort Ngamphiw, Chumpol
collection PubMed
description Long INterspersed Element-1 (LINE-1 or L1) is a retrotransposable element that has shaped the evolution of mammalian genomes. There is increasing evidence that transcriptionally active L1 could have been co-opted through evolution to play various roles including X-inactivation, homologous recombination and gene regulation. Here, we compare putatively active L1 distributions in the mouse with human. L1 density is higher in the mouse except for the Y-chromosome. L1 density is the highest in X-chromosome, implying an X-inactivation role. L1 is more common outside genes (intergenic) except for the Y-chromosome in both species. The structure of mouse L1 is distinguished from human L1 by the presence of a 200 bp repeat in the 5′ UTR of the former. We found that mouse intragenic L1 has significantly higher repeat copy numbers than intergenic L1, suggesting that this is important for control of L1 expression. Furthermore, a significant association between the presence of intragenic L1s and down-regulated genes in early embryogenesis was found in both species. In conclusion, the distribution of L1 in the mouse genome points to biological roles of L1 in mouse similar to human.
format Online
Article
Text
id pubmed-4237456
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-42374562014-11-21 Roles of Intragenic and Intergenic L1s in Mouse and Human Ngamphiw, Chumpol Tongsima, Sissades Mutirangura, Apiwat PLoS One Research Article Long INterspersed Element-1 (LINE-1 or L1) is a retrotransposable element that has shaped the evolution of mammalian genomes. There is increasing evidence that transcriptionally active L1 could have been co-opted through evolution to play various roles including X-inactivation, homologous recombination and gene regulation. Here, we compare putatively active L1 distributions in the mouse with human. L1 density is higher in the mouse except for the Y-chromosome. L1 density is the highest in X-chromosome, implying an X-inactivation role. L1 is more common outside genes (intergenic) except for the Y-chromosome in both species. The structure of mouse L1 is distinguished from human L1 by the presence of a 200 bp repeat in the 5′ UTR of the former. We found that mouse intragenic L1 has significantly higher repeat copy numbers than intergenic L1, suggesting that this is important for control of L1 expression. Furthermore, a significant association between the presence of intragenic L1s and down-regulated genes in early embryogenesis was found in both species. In conclusion, the distribution of L1 in the mouse genome points to biological roles of L1 in mouse similar to human. Public Library of Science 2014-11-19 /pmc/articles/PMC4237456/ /pubmed/25409429 http://dx.doi.org/10.1371/journal.pone.0113434 Text en © 2014 Ngamphiw et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ngamphiw, Chumpol
Tongsima, Sissades
Mutirangura, Apiwat
Roles of Intragenic and Intergenic L1s in Mouse and Human
title Roles of Intragenic and Intergenic L1s in Mouse and Human
title_full Roles of Intragenic and Intergenic L1s in Mouse and Human
title_fullStr Roles of Intragenic and Intergenic L1s in Mouse and Human
title_full_unstemmed Roles of Intragenic and Intergenic L1s in Mouse and Human
title_short Roles of Intragenic and Intergenic L1s in Mouse and Human
title_sort roles of intragenic and intergenic l1s in mouse and human
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237456/
https://www.ncbi.nlm.nih.gov/pubmed/25409429
http://dx.doi.org/10.1371/journal.pone.0113434
work_keys_str_mv AT ngamphiwchumpol rolesofintragenicandintergenicl1sinmouseandhuman
AT tongsimasissades rolesofintragenicandintergenicl1sinmouseandhuman
AT mutiranguraapiwat rolesofintragenicandintergenicl1sinmouseandhuman