Cargando…
Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles
BACKGROUND: Recently, non-thermal atmospheric pressure plasma sources have been used for biomedical applications such as sterilization, cancer treatment, blood coagulation, and wound healing. Gold nanoparticles (gNPs) have unique optical properties and are useful for biomedical applications. Althoug...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237858/ https://www.ncbi.nlm.nih.gov/pubmed/25104171 http://dx.doi.org/10.1186/s12951-014-0029-5 |
_version_ | 1782345411915153408 |
---|---|
author | Park, Sang Rye Lee, Hyun Wook Hong, Jin Woo Lee, Hae June Kim, Ji Young Choi, Byul bo-ra Kim, Gyoo Cheon Jeon, Young Chan |
author_facet | Park, Sang Rye Lee, Hyun Wook Hong, Jin Woo Lee, Hae June Kim, Ji Young Choi, Byul bo-ra Kim, Gyoo Cheon Jeon, Young Chan |
author_sort | Park, Sang Rye |
collection | PubMed |
description | BACKGROUND: Recently, non-thermal atmospheric pressure plasma sources have been used for biomedical applications such as sterilization, cancer treatment, blood coagulation, and wound healing. Gold nanoparticles (gNPs) have unique optical properties and are useful for biomedical applications. Although low-temperature plasma has been shown to be effective in killing oral bacteria on agar plates, its bactericidal effect is negligible on the tooth surface. Therefore, we used 30-nm gNPs to enhance the killing effect of low-temperature plasma on human teeth. RESULTS: We tested the sterilizing effect of low-temperature plasma on Streptococcus mutans (S. mutans) strains. The survival rate was assessed by bacterial viability stains and colony-forming unit counts. Low-temperature plasma treatment alone was effective in killing S. mutans on slide glasses, as shown by the 5-log decrease in viability. However, plasma treatment of bacteria spotted onto tooth surface exhibited a 3-log reduction in viability. After gNPs were added to S. mutans, plasma treatment caused a 5-log reduction in viability, while gNPs alone did not show any bactericidal effect. The morphological changes in S. mutans caused by plasma treatment were examined by transmission electron microscopy, which showed that plasma treatment only perforated the cell walls, while the combination treatment with plasma and gold nanoparticles caused significant cell rupture, causing loss of intracellular components from many cells. CONCLUSIONS: This study demonstrates that low-temperature plasma treatment is effective in killing S. mutans and that its killing effect is further enhanced when used in combination with gNPs. |
format | Online Article Text |
id | pubmed-4237858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42378582014-11-21 Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles Park, Sang Rye Lee, Hyun Wook Hong, Jin Woo Lee, Hae June Kim, Ji Young Choi, Byul bo-ra Kim, Gyoo Cheon Jeon, Young Chan J Nanobiotechnology Research BACKGROUND: Recently, non-thermal atmospheric pressure plasma sources have been used for biomedical applications such as sterilization, cancer treatment, blood coagulation, and wound healing. Gold nanoparticles (gNPs) have unique optical properties and are useful for biomedical applications. Although low-temperature plasma has been shown to be effective in killing oral bacteria on agar plates, its bactericidal effect is negligible on the tooth surface. Therefore, we used 30-nm gNPs to enhance the killing effect of low-temperature plasma on human teeth. RESULTS: We tested the sterilizing effect of low-temperature plasma on Streptococcus mutans (S. mutans) strains. The survival rate was assessed by bacterial viability stains and colony-forming unit counts. Low-temperature plasma treatment alone was effective in killing S. mutans on slide glasses, as shown by the 5-log decrease in viability. However, plasma treatment of bacteria spotted onto tooth surface exhibited a 3-log reduction in viability. After gNPs were added to S. mutans, plasma treatment caused a 5-log reduction in viability, while gNPs alone did not show any bactericidal effect. The morphological changes in S. mutans caused by plasma treatment were examined by transmission electron microscopy, which showed that plasma treatment only perforated the cell walls, while the combination treatment with plasma and gold nanoparticles caused significant cell rupture, causing loss of intracellular components from many cells. CONCLUSIONS: This study demonstrates that low-temperature plasma treatment is effective in killing S. mutans and that its killing effect is further enhanced when used in combination with gNPs. BioMed Central 2014-08-08 /pmc/articles/PMC4237858/ /pubmed/25104171 http://dx.doi.org/10.1186/s12951-014-0029-5 Text en Copyright © 2014 Park et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Park, Sang Rye Lee, Hyun Wook Hong, Jin Woo Lee, Hae June Kim, Ji Young Choi, Byul bo-ra Kim, Gyoo Cheon Jeon, Young Chan Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles |
title | Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles |
title_full | Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles |
title_fullStr | Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles |
title_full_unstemmed | Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles |
title_short | Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles |
title_sort | enhancement of the killing effect of low-temperature plasma on streptococcus mutans by combined treatment with gold nanoparticles |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237858/ https://www.ncbi.nlm.nih.gov/pubmed/25104171 http://dx.doi.org/10.1186/s12951-014-0029-5 |
work_keys_str_mv | AT parksangrye enhancementofthekillingeffectoflowtemperatureplasmaonstreptococcusmutansbycombinedtreatmentwithgoldnanoparticles AT leehyunwook enhancementofthekillingeffectoflowtemperatureplasmaonstreptococcusmutansbycombinedtreatmentwithgoldnanoparticles AT hongjinwoo enhancementofthekillingeffectoflowtemperatureplasmaonstreptococcusmutansbycombinedtreatmentwithgoldnanoparticles AT leehaejune enhancementofthekillingeffectoflowtemperatureplasmaonstreptococcusmutansbycombinedtreatmentwithgoldnanoparticles AT kimjiyoung enhancementofthekillingeffectoflowtemperatureplasmaonstreptococcusmutansbycombinedtreatmentwithgoldnanoparticles AT choibyulbora enhancementofthekillingeffectoflowtemperatureplasmaonstreptococcusmutansbycombinedtreatmentwithgoldnanoparticles AT kimgyoocheon enhancementofthekillingeffectoflowtemperatureplasmaonstreptococcusmutansbycombinedtreatmentwithgoldnanoparticles AT jeonyoungchan enhancementofthekillingeffectoflowtemperatureplasmaonstreptococcusmutansbycombinedtreatmentwithgoldnanoparticles |