Development of a hexahistidine-3× FLAG-tandem affinity purification method for endogenous protein complexes in Pichia pastoris

We developed a method for efficient chromosome tagging in Pichia pastoris, using a useful tandem affinity purification (TAP) tag. The TAP tag, designated and used here as the THF tag, contains a thrombin protease cleavage site for removal of the TAP tag and a hexahistidine sequence (6× His) followed...

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Detalles Bibliográficos
Autores principales: Higo, Toshiaki, Suka, Noriyuki, Ehara, Haruhiko, Wakamori, Masatoshi, Sato, Shin, Maeda, Hideaki, Sekine, Shun-ichi, Umehara, Takashi, Yokoyama, Shigeyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237914/
https://www.ncbi.nlm.nih.gov/pubmed/25398586
http://dx.doi.org/10.1007/s10969-014-9190-1

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