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Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation
Endoplasmic reticulum (ER) is motile within dendritic spines, but the mechanisms underlying its regulation are poorly understood. To address this issue, we have simultaneously imaged morphology and ER content of dendritic spines in cultured dissociated mouse hippocampal neurons. Over a 10 min period...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237958/ https://www.ncbi.nlm.nih.gov/pubmed/25242397 http://dx.doi.org/10.1186/s13041-014-0060-3 |
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author | Ng, Ai Na Doherty, Andrew J Lombroso, Paul J Emptage, Nigel J Collingridge, Graham L |
author_facet | Ng, Ai Na Doherty, Andrew J Lombroso, Paul J Emptage, Nigel J Collingridge, Graham L |
author_sort | Ng, Ai Na |
collection | PubMed |
description | Endoplasmic reticulum (ER) is motile within dendritic spines, but the mechanisms underlying its regulation are poorly understood. To address this issue, we have simultaneously imaged morphology and ER content of dendritic spines in cultured dissociated mouse hippocampal neurons. Over a 10 min period, spines were highly dynamic, with spines both increasing and decreasing in volume. ER was present in approximately 50% of spines and was also highly dynamic, with a net increase over this period of time. Inhibition of the endogenous activation of NMDA receptors resulted in a reduction in ER growth. Conversely, augmentation of the synaptic activation of NMDA receptors, by elimination of striatal-enriched protein tyrosine phosphatase (STEP), resulted in enhanced ER growth. Therefore, NMDA receptors rapidly regulate spine ER dynamics. |
format | Online Article Text |
id | pubmed-4237958 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42379582014-11-21 Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation Ng, Ai Na Doherty, Andrew J Lombroso, Paul J Emptage, Nigel J Collingridge, Graham L Mol Brain Research Endoplasmic reticulum (ER) is motile within dendritic spines, but the mechanisms underlying its regulation are poorly understood. To address this issue, we have simultaneously imaged morphology and ER content of dendritic spines in cultured dissociated mouse hippocampal neurons. Over a 10 min period, spines were highly dynamic, with spines both increasing and decreasing in volume. ER was present in approximately 50% of spines and was also highly dynamic, with a net increase over this period of time. Inhibition of the endogenous activation of NMDA receptors resulted in a reduction in ER growth. Conversely, augmentation of the synaptic activation of NMDA receptors, by elimination of striatal-enriched protein tyrosine phosphatase (STEP), resulted in enhanced ER growth. Therefore, NMDA receptors rapidly regulate spine ER dynamics. BioMed Central 2014-08-19 /pmc/articles/PMC4237958/ /pubmed/25242397 http://dx.doi.org/10.1186/s13041-014-0060-3 Text en Copyright © 2014 Ng et al. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ng, Ai Na Doherty, Andrew J Lombroso, Paul J Emptage, Nigel J Collingridge, Graham L Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation |
title | Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation |
title_full | Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation |
title_fullStr | Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation |
title_full_unstemmed | Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation |
title_short | Rapid regulation of endoplasmic reticulum dynamics in dendritic spines by NMDA receptor activation |
title_sort | rapid regulation of endoplasmic reticulum dynamics in dendritic spines by nmda receptor activation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237958/ https://www.ncbi.nlm.nih.gov/pubmed/25242397 http://dx.doi.org/10.1186/s13041-014-0060-3 |
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