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Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations

Non-thermal plasma has been recognized as a promising tool across a vast variety of biomedical applications, with the potential to create novel therapeutic methods. However, the understanding of the molecular mechanisms behind non-thermal plasma cellular effects remains a significant challenge. In t...

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Autores principales: Lunov, Oleg, Zablotskii, Vitalii, Churpita, Olexander, Chánová, Eliška, Syková, Eva, Dejneka, Alexandr, Kubinová, Šárka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238021/
https://www.ncbi.nlm.nih.gov/pubmed/25410636
http://dx.doi.org/10.1038/srep07129
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author Lunov, Oleg
Zablotskii, Vitalii
Churpita, Olexander
Chánová, Eliška
Syková, Eva
Dejneka, Alexandr
Kubinová, Šárka
author_facet Lunov, Oleg
Zablotskii, Vitalii
Churpita, Olexander
Chánová, Eliška
Syková, Eva
Dejneka, Alexandr
Kubinová, Šárka
author_sort Lunov, Oleg
collection PubMed
description Non-thermal plasma has been recognized as a promising tool across a vast variety of biomedical applications, with the potential to create novel therapeutic methods. However, the understanding of the molecular mechanisms behind non-thermal plasma cellular effects remains a significant challenge. In this study, we show how two types of different non-thermal plasmas induce cell death in mammalian cell cultures via the formation of multiple intracellular reactive oxygen/nitrogen species. Our results showed a discrepancy in the superoxide accumulation and lysosomal activity in response to air and helium plasma, suggesting that triggered signalling cascades might be grossly different between different plasmas. In addition, the effects of ozone, a considerable component of non-thermal plasma, have been simultaneously evaluated and have revealed much faster and higher cytotoxic effects. Our findings offer novel insight into plasma-induced cellular responses, and provide a basis for better controlled biomedical applications.
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spelling pubmed-42380212014-11-25 Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations Lunov, Oleg Zablotskii, Vitalii Churpita, Olexander Chánová, Eliška Syková, Eva Dejneka, Alexandr Kubinová, Šárka Sci Rep Article Non-thermal plasma has been recognized as a promising tool across a vast variety of biomedical applications, with the potential to create novel therapeutic methods. However, the understanding of the molecular mechanisms behind non-thermal plasma cellular effects remains a significant challenge. In this study, we show how two types of different non-thermal plasmas induce cell death in mammalian cell cultures via the formation of multiple intracellular reactive oxygen/nitrogen species. Our results showed a discrepancy in the superoxide accumulation and lysosomal activity in response to air and helium plasma, suggesting that triggered signalling cascades might be grossly different between different plasmas. In addition, the effects of ozone, a considerable component of non-thermal plasma, have been simultaneously evaluated and have revealed much faster and higher cytotoxic effects. Our findings offer novel insight into plasma-induced cellular responses, and provide a basis for better controlled biomedical applications. Nature Publishing Group 2014-11-20 /pmc/articles/PMC4238021/ /pubmed/25410636 http://dx.doi.org/10.1038/srep07129 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lunov, Oleg
Zablotskii, Vitalii
Churpita, Olexander
Chánová, Eliška
Syková, Eva
Dejneka, Alexandr
Kubinová, Šárka
Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
title Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
title_full Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
title_fullStr Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
title_full_unstemmed Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
title_short Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
title_sort cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238021/
https://www.ncbi.nlm.nih.gov/pubmed/25410636
http://dx.doi.org/10.1038/srep07129
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