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Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
Non-thermal plasma has been recognized as a promising tool across a vast variety of biomedical applications, with the potential to create novel therapeutic methods. However, the understanding of the molecular mechanisms behind non-thermal plasma cellular effects remains a significant challenge. In t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238021/ https://www.ncbi.nlm.nih.gov/pubmed/25410636 http://dx.doi.org/10.1038/srep07129 |
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author | Lunov, Oleg Zablotskii, Vitalii Churpita, Olexander Chánová, Eliška Syková, Eva Dejneka, Alexandr Kubinová, Šárka |
author_facet | Lunov, Oleg Zablotskii, Vitalii Churpita, Olexander Chánová, Eliška Syková, Eva Dejneka, Alexandr Kubinová, Šárka |
author_sort | Lunov, Oleg |
collection | PubMed |
description | Non-thermal plasma has been recognized as a promising tool across a vast variety of biomedical applications, with the potential to create novel therapeutic methods. However, the understanding of the molecular mechanisms behind non-thermal plasma cellular effects remains a significant challenge. In this study, we show how two types of different non-thermal plasmas induce cell death in mammalian cell cultures via the formation of multiple intracellular reactive oxygen/nitrogen species. Our results showed a discrepancy in the superoxide accumulation and lysosomal activity in response to air and helium plasma, suggesting that triggered signalling cascades might be grossly different between different plasmas. In addition, the effects of ozone, a considerable component of non-thermal plasma, have been simultaneously evaluated and have revealed much faster and higher cytotoxic effects. Our findings offer novel insight into plasma-induced cellular responses, and provide a basis for better controlled biomedical applications. |
format | Online Article Text |
id | pubmed-4238021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-42380212014-11-25 Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations Lunov, Oleg Zablotskii, Vitalii Churpita, Olexander Chánová, Eliška Syková, Eva Dejneka, Alexandr Kubinová, Šárka Sci Rep Article Non-thermal plasma has been recognized as a promising tool across a vast variety of biomedical applications, with the potential to create novel therapeutic methods. However, the understanding of the molecular mechanisms behind non-thermal plasma cellular effects remains a significant challenge. In this study, we show how two types of different non-thermal plasmas induce cell death in mammalian cell cultures via the formation of multiple intracellular reactive oxygen/nitrogen species. Our results showed a discrepancy in the superoxide accumulation and lysosomal activity in response to air and helium plasma, suggesting that triggered signalling cascades might be grossly different between different plasmas. In addition, the effects of ozone, a considerable component of non-thermal plasma, have been simultaneously evaluated and have revealed much faster and higher cytotoxic effects. Our findings offer novel insight into plasma-induced cellular responses, and provide a basis for better controlled biomedical applications. Nature Publishing Group 2014-11-20 /pmc/articles/PMC4238021/ /pubmed/25410636 http://dx.doi.org/10.1038/srep07129 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Lunov, Oleg Zablotskii, Vitalii Churpita, Olexander Chánová, Eliška Syková, Eva Dejneka, Alexandr Kubinová, Šárka Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations |
title | Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations |
title_full | Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations |
title_fullStr | Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations |
title_full_unstemmed | Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations |
title_short | Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations |
title_sort | cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238021/ https://www.ncbi.nlm.nih.gov/pubmed/25410636 http://dx.doi.org/10.1038/srep07129 |
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