Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography

Advances in electron cryo-tomography open up a new avenue to visualize the 3-D internal structure of a single bacterium before and after its infection by bacteriophages in its native environment, without using chemical fixatives, fluorescent dyes or negative stains. Such direct observation reveals t...

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Detalles Bibliográficos
Autores principales: Dai, Wei, Schmid, Michael F., King, Jonathan A., Chiu, Wah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shared Science Publishers OG 2014
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238041/
https://www.ncbi.nlm.nih.gov/pubmed/25419524
http://dx.doi.org/10.15698/mic2014.01.125
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author Dai, Wei
Schmid, Michael F.
King, Jonathan A.
Chiu, Wah
author_facet Dai, Wei
Schmid, Michael F.
King, Jonathan A.
Chiu, Wah
author_sort Dai, Wei
collection PubMed
description Advances in electron cryo-tomography open up a new avenue to visualize the 3-D internal structure of a single bacterium before and after its infection by bacteriophages in its native environment, without using chemical fixatives, fluorescent dyes or negative stains. Such direct observation reveals the presence of assembly intermediates of the bacteriophage and thus allows us to map out the maturation pathway of the bacteriophage inside its host.
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spelling pubmed-42380412014-11-20 Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography Dai, Wei Schmid, Michael F. King, Jonathan A. Chiu, Wah Microb Cell Microbiology Advances in electron cryo-tomography open up a new avenue to visualize the 3-D internal structure of a single bacterium before and after its infection by bacteriophages in its native environment, without using chemical fixatives, fluorescent dyes or negative stains. Such direct observation reveals the presence of assembly intermediates of the bacteriophage and thus allows us to map out the maturation pathway of the bacteriophage inside its host. Shared Science Publishers OG 2014-01-06 /pmc/articles/PMC4238041/ /pubmed/25419524 http://dx.doi.org/10.15698/mic2014.01.125 Text en https://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial Share Alike License, which permits the copy and distribution of the unmodified material in any medium or format, provided the original work is properly cited and the material not used for commercial purposes. If the material is remixed, transformed or build upon, the modified material may not be distributed.
spellingShingle Microbiology
Dai, Wei
Schmid, Michael F.
King, Jonathan A.
Chiu, Wah
Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography
title Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography
title_full Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography
title_fullStr Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography
title_full_unstemmed Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography
title_short Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography
title_sort identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238041/
https://www.ncbi.nlm.nih.gov/pubmed/25419524
http://dx.doi.org/10.15698/mic2014.01.125
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