Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil

Mayaro virus (MAYV) is frequently reported in Pan-Amazonia. The aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of Mato Grosso, Brazil. Serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptas...

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Autores principales: Zuchi, Nayara, Heinen, Letícia Borges da Silva, dos Santos, Marcelo Adriano Mendes, Pereira, Fernanda Carla, Slhessarenko, Renata Dezengrini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238776/
https://www.ncbi.nlm.nih.gov/pubmed/25141284
http://dx.doi.org/10.1590/0074-0276140108
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author Zuchi, Nayara
Heinen, Letícia Borges da Silva
dos Santos, Marcelo Adriano Mendes
Pereira, Fernanda Carla
Slhessarenko, Renata Dezengrini
author_facet Zuchi, Nayara
Heinen, Letícia Borges da Silva
dos Santos, Marcelo Adriano Mendes
Pereira, Fernanda Carla
Slhessarenko, Renata Dezengrini
author_sort Zuchi, Nayara
collection PubMed
description Mayaro virus (MAYV) is frequently reported in Pan-Amazonia. The aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of Mato Grosso, Brazil. Serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alphaviruses, to nucleotide sequencing and to viral isolation. MAYV was detected in 15 (2.5%) of 604 patients. Twelve were co-infected with dengue virus 4, which was isolated from 10 patients. The molecular detection of MAYV in dengue-suspected patients suggests that other arboviruses may be silently circulating during dengue outbreaks in Brazil.
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spelling pubmed-42387762014-11-26 Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil Zuchi, Nayara Heinen, Letícia Borges da Silva dos Santos, Marcelo Adriano Mendes Pereira, Fernanda Carla Slhessarenko, Renata Dezengrini Mem Inst Oswaldo Cruz Short Communication Mayaro virus (MAYV) is frequently reported in Pan-Amazonia. The aim of this study was to investigate the circulation of alphaviruses during a dengue outbreak in the state of Mato Grosso, Brazil. Serum samples from dengue-suspected patients were subjected to multiplex semi-nested reverse transcriptase polymerase chain reaction for 11 flaviviruses and five alphaviruses, to nucleotide sequencing and to viral isolation. MAYV was detected in 15 (2.5%) of 604 patients. Twelve were co-infected with dengue virus 4, which was isolated from 10 patients. The molecular detection of MAYV in dengue-suspected patients suggests that other arboviruses may be silently circulating during dengue outbreaks in Brazil. Instituto Oswaldo Cruz, Ministério da Saúde 2014-09 /pmc/articles/PMC4238776/ /pubmed/25141284 http://dx.doi.org/10.1590/0074-0276140108 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Zuchi, Nayara
Heinen, Letícia Borges da Silva
dos Santos, Marcelo Adriano Mendes
Pereira, Fernanda Carla
Slhessarenko, Renata Dezengrini
Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil
title Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil
title_full Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil
title_fullStr Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil
title_full_unstemmed Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil
title_short Molecular detection of Mayaro virus during a dengue outbreak in the state of Mato Grosso, Central-West Brazil
title_sort molecular detection of mayaro virus during a dengue outbreak in the state of mato grosso, central-west brazil
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4238776/
https://www.ncbi.nlm.nih.gov/pubmed/25141284
http://dx.doi.org/10.1590/0074-0276140108
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