Cargando…

A comparison of commercially-available automated and manual extraction kits for the isolation of total RNA from small tissue samples

BACKGROUND: This study compared the performance of five commercially available kits in extracting total RNA from small eukaryotic tissue samples (<15 mg). Total RNA was isolated from fathead minnow (Pimephales promelas) tissues (spleen, blood, kidney, embryo, and larvae) using the Qiagen RNeasy®...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sellin Jeffries, Marlo K, Kiss, Andor J, Smith, Austin W, Oris, James T
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4239376/ https://www.ncbi.nlm.nih.gov/pubmed/25394494 http://dx.doi.org/10.1186/s12896-014-0094-8

Ejemplares similares

Evaluation of commercially available small RNASeq library preparation kits using low input RNA
por: Yeri, Ashish, et al.
Publicado: (2018)

Validation of dried blood spot sample modifications to two commercially available COVID-19 IgG antibody immunoassays
por: Zava, Theodore T, et al.
Publicado: (2020)

DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits
por: Kovacs, Zsolt, et al.
Publicado: (2019)

Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits
por: Gervasoni, Jacopo, et al.
Publicado: (2013)

Comparison of commercial kits to measure cytokine responses to Plasmodium falciparum by multiplex microsphere suspension array technology
por: Berthoud, Tamara K, et al.
Publicado: (2011)

Comparative evaluation of commercially available manual and automated nucleic acid extraction methods for rotavirus RNA detection in stools
por: Esona, Mathew D., et al.
Publicado: (2013)

Evaluation of commercially available DNA extraction kits for the analysis of the broiler chicken cecal microbiota
por: Pankoke, Helga, et al.
Publicado: (2019)

Evaluation of two commercial kits and two laboratory-developed qPCR assays compared to LAMP for molecular diagnosis of malaria
por: Bouzayene, Azza, et al.
Publicado: (2022)

MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits
por: Fromm, Bastian, et al.
Publicado: (2011)

Usefulness of commercially available GPS data-loggers for tracking human movement and exposure to dengue virus
por: Vazquez-Prokopec, Gonzalo M, et al.
Publicado: (2009)

Visualization of zinc dynamics in intact plants using positron imaging of commercially available (65)Zn
por: Suzui, Nobuo, et al.
Publicado: (2017)

Use of a recombinase polymerase amplification commercial kit for rapid visual detection of Pasteurella multocida
por: Zhao, Guimin, et al.
Publicado: (2019)

Purchasing online journal access for a hospital medical library: how to identify value in commercially available products
por: Carter, Thomas P, et al.
Publicado: (2006)

Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum
por: Li, Xiaolin, et al.
Publicado: (2019)

Automated vs manual delineations of regions of interest- a comparison in commercially available perfusion MRI software
por: Galinovic, Ivana, et al.
Publicado: (2012)

Comparison of Two Commercially Available qPCR Kits for the Detection of Candida auris
por: Sattler, Janko, et al.
Publicado: (2021)

Comparison of the Effectiveness of Four Commercial DNA Extraction Kits on Fresh and Frozen Human Milk Samples
por: Butler, Cassidy, et al.
Publicado: (2022)

A comparison of the efficiency of five different commercial DNA extraction kits for extraction of DNA from faecal samples
por: Claassen, Shantelle, et al.
Publicado: (2013)

Comparison of Modified Manual Acid-Phenol Chloroform Method and Commercial RNA Extraction Kits for Resource Limited Laboratories
por: Sakyi, Samuel Asamoah, et al.
Publicado: (2023)

ciliaFA: a research tool for automated, high-throughput measurement of ciliary beat frequency using freely available software
por: Smith, Claire M, et al.
Publicado: (2012)

Automated extraction of chemical structure information from digital raster images
por: Park, Jungkap, et al.
Publicado: (2009)

Diagnostic Accuracy of Commercially Available Automated External Defibrillators
por: Nishiyama, Takahiko, et al.
Publicado: (2015)

Comparison of commercial RNA extraction kits for preparation of DNA-free total RNA from Salmonella cells
por: Rump, Lydia V, et al.
Publicado: (2010)

ARAM: an automated image analysis software to determine rosetting parameters and parasitaemia in Plasmodium samples
por: Kudella, Patrick Wolfgang, et al.
Publicado: (2016)

Automated macrophage counting in DLBCL tissue samples: a ROF filter based approach
por: Wagner, Marcus, et al.
Publicado: (2019)

Systematic assessment of commercially available low-input miRNA library preparation kits
por: Heinicke, Fatima, et al.
Publicado: (2019)

Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle
por: Munita, Maria Pia, et al.
Publicado: (2019)

Supervised learning for the automated transcription of spacer classification from spoligotype films
por: Jeffries, David J, et al.
Publicado: (2009)

A practical guide for assessing respiratory burst and phagocytic cell activity in the fathead minnow, an emerging model for immunotoxicity
por: Hampton, Leah M. Thornton, et al.
Publicado: (2020)

Can rapid dengue diagnostic kits be trusted? A comparative study of commercially available rapid kits for serodiagnosis of dengue fever
por: Garg, Atul, et al.
Publicado: (2019)

Rapid methods to detect organic mercury and total selenium in biological samples
por: Nam, Dong-Ha, et al.
Publicado: (2011)

A new ELISA kit which uses a combination of Plasmodium falciparum extract and recombinant Plasmodium vivax antigens as an alternative to IFAT for detection of malaria antibodies
por: Doderer, Cecile, et al.
Publicado: (2007)

SEEMLIS: a flexible semi-automated method for enrichment of methylated DNA from low-input samples
por: Rodems, Tamara S., et al.
Publicado: (2022)

Comparison of commercial DNA extraction kits for whole metagenome sequencing of human oral, vaginal, and rectal microbiome samples
por: Wright, Michelle L., et al.
Publicado: (2023)

Diagnostic Performance of Commercially Available Enzyme-Linked Immunosorbent Assay Kit in the Diagnosis of Extrapulmonary Tuberculosis
por: Sankar, Manimuthu Mani, et al.
Publicado: (2013)

An extension to: Systematic assessment of commercially available low-input miRNA library preparation kits
por: Heinicke, Fatima, et al.
Publicado: (2020)

Simple extraction methods that prevent the artifactual conversion of chlorophyll to chlorophyllide during pigment isolation from leaf samples
por: Hu, Xueyun, et al.
Publicado: (2013)

A comparison of four commercially available RNA extraction kits for wastewater surveillance of SARS-CoV-2 in a college population
por: O'Brien, Megan, et al.
Publicado: (2021)

Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions
por: Lee, Eun Young, et al.
Publicado: (2020)

Evaluation of a prototype machine learning tool to semi-automate data extraction for systematic literature reviews
por: Panayi, Antonia, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_r64ta510hlgm650eticfhhk1qn