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Simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum
BACKGROUND: Cervi Cornu Pantotrichum has been a well known traditional Chinese medicine, which is young horn of Cervus Nippon Temminck (Hualurong: HLR). At present, the methods used for the quality control of Cervi Cornu Pantotrichum show low specificity. OBJECTIVE: To describe a holistic method bas...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4239713/ https://www.ncbi.nlm.nih.gov/pubmed/25422536 http://dx.doi.org/10.4103/0973-1296.141757 |
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author | Zong, Ying Wang, Yu Li, Hang Li, Na Zhang, Hui Sun, Jiaming Niu, Xiaohui Gao, Xiaochen |
author_facet | Zong, Ying Wang, Yu Li, Hang Li, Na Zhang, Hui Sun, Jiaming Niu, Xiaohui Gao, Xiaochen |
author_sort | Zong, Ying |
collection | PubMed |
description | BACKGROUND: Cervi Cornu Pantotrichum has been a well known traditional Chinese medicine, which is young horn of Cervus Nippon Temminck (Hualurong: HLR). At present, the methods used for the quality control of Cervi Cornu Pantotrichum show low specificity. OBJECTIVE: To describe a holistic method based on chemical characteristics and splenocyte-proliferating activities to evaluate the quality of HLR. MATERIALS AND METHODS: The nucleosides and bases from HLR were identified by high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS), and six of them were chosen to be used for simultaneous HPLC quantification according to the results of proliferation of mouse splenocytes in vitro. RESULTS: In this study, eight nucleosides and bases have been identified. In addition, uracil, hypoxanthine, uridine, inosine, guanosine, and adenosine were chosen to be used for simultaneous HPLC quantification. Simultaneous quantification of these six substances was performed on ten groups of HLR under the condition of a TIANHE Kromasil C(18) column (5 μm, 4.6 mm × 250 mm i.d.) and a gradient elution of water and acetonitrile. Of the ten groups, HLR displayed the highest total nucleoside contents (TNC, sum of adenosine and uracil, 0.412 mg/g) with the strongest splenocyte-proliferating activities. CONCLUSION: These results suggest that TNC (such as particularly highly contained adenosine and uracil) in HLR has a certain correlation with the activity of splenocyte-proliferating, and it may be used as a quality control for HLR. This comprehensive method could be applied to other traditional Chinese medicines to ameliorate their quality control. |
format | Online Article Text |
id | pubmed-4239713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42397132014-11-24 Simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum Zong, Ying Wang, Yu Li, Hang Li, Na Zhang, Hui Sun, Jiaming Niu, Xiaohui Gao, Xiaochen Pharmacogn Mag Original Article BACKGROUND: Cervi Cornu Pantotrichum has been a well known traditional Chinese medicine, which is young horn of Cervus Nippon Temminck (Hualurong: HLR). At present, the methods used for the quality control of Cervi Cornu Pantotrichum show low specificity. OBJECTIVE: To describe a holistic method based on chemical characteristics and splenocyte-proliferating activities to evaluate the quality of HLR. MATERIALS AND METHODS: The nucleosides and bases from HLR were identified by high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS), and six of them were chosen to be used for simultaneous HPLC quantification according to the results of proliferation of mouse splenocytes in vitro. RESULTS: In this study, eight nucleosides and bases have been identified. In addition, uracil, hypoxanthine, uridine, inosine, guanosine, and adenosine were chosen to be used for simultaneous HPLC quantification. Simultaneous quantification of these six substances was performed on ten groups of HLR under the condition of a TIANHE Kromasil C(18) column (5 μm, 4.6 mm × 250 mm i.d.) and a gradient elution of water and acetonitrile. Of the ten groups, HLR displayed the highest total nucleoside contents (TNC, sum of adenosine and uracil, 0.412 mg/g) with the strongest splenocyte-proliferating activities. CONCLUSION: These results suggest that TNC (such as particularly highly contained adenosine and uracil) in HLR has a certain correlation with the activity of splenocyte-proliferating, and it may be used as a quality control for HLR. This comprehensive method could be applied to other traditional Chinese medicines to ameliorate their quality control. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4239713/ /pubmed/25422536 http://dx.doi.org/10.4103/0973-1296.141757 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zong, Ying Wang, Yu Li, Hang Li, Na Zhang, Hui Sun, Jiaming Niu, Xiaohui Gao, Xiaochen Simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum |
title | Simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum |
title_full | Simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum |
title_fullStr | Simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum |
title_full_unstemmed | Simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum |
title_short | Simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in Cervi cornu Pantotrichum |
title_sort | simultaneous quantification and splenocyte-proliferating activities of nucleosides and bases in cervi cornu pantotrichum |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4239713/ https://www.ncbi.nlm.nih.gov/pubmed/25422536 http://dx.doi.org/10.4103/0973-1296.141757 |
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