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Expression of genes encoding extracellular matrix proteins: A macroarray study
Endometrial cancer (EC) is one of the most common gynecological malignancies in Poland, with well-established risk factors. Genetic instability and molecular alterations responsible for endometrial carcinogenesis have been systematically investigated. The aim of the present study was to investigate,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240474/ https://www.ncbi.nlm.nih.gov/pubmed/25231141 http://dx.doi.org/10.3892/or.2014.3493 |
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author | FUTYMA, KONRAD MIOTŁA, PAWEŁ RÓŻYŃSKA, KRYSTYNA ZDUNEK, MAŁGORZATA SEMCZUK, ANDRZEJ RECHBERGER, TOMASZ WOJCIEROWSKI, JACEK |
author_facet | FUTYMA, KONRAD MIOTŁA, PAWEŁ RÓŻYŃSKA, KRYSTYNA ZDUNEK, MAŁGORZATA SEMCZUK, ANDRZEJ RECHBERGER, TOMASZ WOJCIEROWSKI, JACEK |
author_sort | FUTYMA, KONRAD |
collection | PubMed |
description | Endometrial cancer (EC) is one of the most common gynecological malignancies in Poland, with well-established risk factors. Genetic instability and molecular alterations responsible for endometrial carcinogenesis have been systematically investigated. The aim of the present study was to investigate, by means of cDNA macroarrays, the expression profiles of genes encoding extracellular matrix (ECM) proteins in ECs. Tissue specimens were collected during surgical procedures from 40 patients with EC, and control tissue was collected from 9 patients with uterine leiomyomas. RNA was isolated and RT-PCR with radioisotope-labeled cDNA was performed. The levels of ECM protein gene expression in normal endometrial tissues were compared to the expression of these genes in EC specimens. Statistically significant differences in gene expression, stratified by clinical stage of the ECs, were detected for aggrecan, vitronectin, tenascin R, nidogen and two collagen proteins: type VIII chain α1 and type XI chain α2. All of these proteins were overexpressed in stage III endometrial carcinomas compared to levels in stage I and II uterine neoplasms. In conclusion, increased expression of genes encoding ECM proteins may play an important role in facilitating accelerated disease progression of human ECs. |
format | Online Article Text |
id | pubmed-4240474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-42404742014-11-21 Expression of genes encoding extracellular matrix proteins: A macroarray study FUTYMA, KONRAD MIOTŁA, PAWEŁ RÓŻYŃSKA, KRYSTYNA ZDUNEK, MAŁGORZATA SEMCZUK, ANDRZEJ RECHBERGER, TOMASZ WOJCIEROWSKI, JACEK Oncol Rep Articles Endometrial cancer (EC) is one of the most common gynecological malignancies in Poland, with well-established risk factors. Genetic instability and molecular alterations responsible for endometrial carcinogenesis have been systematically investigated. The aim of the present study was to investigate, by means of cDNA macroarrays, the expression profiles of genes encoding extracellular matrix (ECM) proteins in ECs. Tissue specimens were collected during surgical procedures from 40 patients with EC, and control tissue was collected from 9 patients with uterine leiomyomas. RNA was isolated and RT-PCR with radioisotope-labeled cDNA was performed. The levels of ECM protein gene expression in normal endometrial tissues were compared to the expression of these genes in EC specimens. Statistically significant differences in gene expression, stratified by clinical stage of the ECs, were detected for aggrecan, vitronectin, tenascin R, nidogen and two collagen proteins: type VIII chain α1 and type XI chain α2. All of these proteins were overexpressed in stage III endometrial carcinomas compared to levels in stage I and II uterine neoplasms. In conclusion, increased expression of genes encoding ECM proteins may play an important role in facilitating accelerated disease progression of human ECs. D.A. Spandidos 2014-12 2014-09-17 /pmc/articles/PMC4240474/ /pubmed/25231141 http://dx.doi.org/10.3892/or.2014.3493 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles FUTYMA, KONRAD MIOTŁA, PAWEŁ RÓŻYŃSKA, KRYSTYNA ZDUNEK, MAŁGORZATA SEMCZUK, ANDRZEJ RECHBERGER, TOMASZ WOJCIEROWSKI, JACEK Expression of genes encoding extracellular matrix proteins: A macroarray study |
title | Expression of genes encoding extracellular matrix proteins: A macroarray study |
title_full | Expression of genes encoding extracellular matrix proteins: A macroarray study |
title_fullStr | Expression of genes encoding extracellular matrix proteins: A macroarray study |
title_full_unstemmed | Expression of genes encoding extracellular matrix proteins: A macroarray study |
title_short | Expression of genes encoding extracellular matrix proteins: A macroarray study |
title_sort | expression of genes encoding extracellular matrix proteins: a macroarray study |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240474/ https://www.ncbi.nlm.nih.gov/pubmed/25231141 http://dx.doi.org/10.3892/or.2014.3493 |
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