Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats

OBJECTIVE(S): To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats. MATERIALS AND METHODS: The rats were divided in 5 groups: control (normotensive) group, Des...

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Autores principales: Athiroh, Nour, Permatasari, Nur, Sargowo, Djanggan, Widodo, M Aris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240798/
https://www.ncbi.nlm.nih.gov/pubmed/25422757
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author Athiroh, Nour
Permatasari, Nur
Sargowo, Djanggan
Widodo, M Aris
author_facet Athiroh, Nour
Permatasari, Nur
Sargowo, Djanggan
Widodo, M Aris
author_sort Athiroh, Nour
collection PubMed
description OBJECTIVE(S): To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats. MATERIALS AND METHODS: The rats were divided in 5 groups: control (normotensive) group, Desoxy cortico sterone (DOCA)-salt hypertensive group, and three DOCA-salt hypertensive groups. All 5 groups received methanolic extract of S. atropurpurea (MESA) at a dosage of 50; 100; and 200 mg/KgBW. Serum nitric oxide (NO) was assayed by colorimetric. Circulating endothelial cells (CECs) and EPCs were assayed using flow cytometry. RESULTS: The administration of MESA(100) and MESA(200) elevated the total plasma nitrate/nitrite levels but cannot reach the level in control group. MESA(100) and MESA(200) also elevated the EPCs number compared with hypertensive group. The administration of MESA significantly (P< 0.05) decreased the CECs number compared to hypertensive groups. CONCLUSION: Methanolic extract of S. atropurpurea is able to modulate total plasma nitrate/nitrite levels and diminish endothelial damage via increasing EPCs.
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spelling pubmed-42407982014-11-24 Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats Athiroh, Nour Permatasari, Nur Sargowo, Djanggan Widodo, M Aris Iran J Basic Med Sci Short Communication OBJECTIVE(S): To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats. MATERIALS AND METHODS: The rats were divided in 5 groups: control (normotensive) group, Desoxy cortico sterone (DOCA)-salt hypertensive group, and three DOCA-salt hypertensive groups. All 5 groups received methanolic extract of S. atropurpurea (MESA) at a dosage of 50; 100; and 200 mg/KgBW. Serum nitric oxide (NO) was assayed by colorimetric. Circulating endothelial cells (CECs) and EPCs were assayed using flow cytometry. RESULTS: The administration of MESA(100) and MESA(200) elevated the total plasma nitrate/nitrite levels but cannot reach the level in control group. MESA(100) and MESA(200) also elevated the EPCs number compared with hypertensive group. The administration of MESA significantly (P< 0.05) decreased the CECs number compared to hypertensive groups. CONCLUSION: Methanolic extract of S. atropurpurea is able to modulate total plasma nitrate/nitrite levels and diminish endothelial damage via increasing EPCs. Mashhad University of Medical Sciences 2014-08 /pmc/articles/PMC4240798/ /pubmed/25422757 Text en © Iranian Journal of Basic Medical Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Athiroh, Nour
Permatasari, Nur
Sargowo, Djanggan
Widodo, M Aris
Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats
title Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats
title_full Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats
title_fullStr Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats
title_full_unstemmed Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats
title_short Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats
title_sort effect of scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of doca-salt hypertensive rats
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240798/
https://www.ncbi.nlm.nih.gov/pubmed/25422757
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