Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats
OBJECTIVE(S): To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats. MATERIALS AND METHODS: The rats were divided in 5 groups: control (normotensive) group, Des...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Mashhad University of Medical Sciences
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240798/ https://www.ncbi.nlm.nih.gov/pubmed/25422757 |
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author | Athiroh, Nour Permatasari, Nur Sargowo, Djanggan Widodo, M Aris |
author_facet | Athiroh, Nour Permatasari, Nur Sargowo, Djanggan Widodo, M Aris |
author_sort | Athiroh, Nour |
collection | PubMed |
description | OBJECTIVE(S): To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats. MATERIALS AND METHODS: The rats were divided in 5 groups: control (normotensive) group, Desoxy cortico sterone (DOCA)-salt hypertensive group, and three DOCA-salt hypertensive groups. All 5 groups received methanolic extract of S. atropurpurea (MESA) at a dosage of 50; 100; and 200 mg/KgBW. Serum nitric oxide (NO) was assayed by colorimetric. Circulating endothelial cells (CECs) and EPCs were assayed using flow cytometry. RESULTS: The administration of MESA(100) and MESA(200) elevated the total plasma nitrate/nitrite levels but cannot reach the level in control group. MESA(100) and MESA(200) also elevated the EPCs number compared with hypertensive group. The administration of MESA significantly (P< 0.05) decreased the CECs number compared to hypertensive groups. CONCLUSION: Methanolic extract of S. atropurpurea is able to modulate total plasma nitrate/nitrite levels and diminish endothelial damage via increasing EPCs. |
format | Online Article Text |
id | pubmed-4240798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-42407982014-11-24 Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats Athiroh, Nour Permatasari, Nur Sargowo, Djanggan Widodo, M Aris Iran J Basic Med Sci Short Communication OBJECTIVE(S): To know whether Scurrula atropurpurea is able to modulate total plasma nitrate/nitrite levels, decrease endothelial damage, and increase endothelial progenitor cells (EPCs) in hypertensive rats. MATERIALS AND METHODS: The rats were divided in 5 groups: control (normotensive) group, Desoxy cortico sterone (DOCA)-salt hypertensive group, and three DOCA-salt hypertensive groups. All 5 groups received methanolic extract of S. atropurpurea (MESA) at a dosage of 50; 100; and 200 mg/KgBW. Serum nitric oxide (NO) was assayed by colorimetric. Circulating endothelial cells (CECs) and EPCs were assayed using flow cytometry. RESULTS: The administration of MESA(100) and MESA(200) elevated the total plasma nitrate/nitrite levels but cannot reach the level in control group. MESA(100) and MESA(200) also elevated the EPCs number compared with hypertensive group. The administration of MESA significantly (P< 0.05) decreased the CECs number compared to hypertensive groups. CONCLUSION: Methanolic extract of S. atropurpurea is able to modulate total plasma nitrate/nitrite levels and diminish endothelial damage via increasing EPCs. Mashhad University of Medical Sciences 2014-08 /pmc/articles/PMC4240798/ /pubmed/25422757 Text en © Iranian Journal of Basic Medical Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Athiroh, Nour Permatasari, Nur Sargowo, Djanggan Widodo, M Aris Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats |
title | Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats |
title_full | Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats |
title_fullStr | Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats |
title_full_unstemmed | Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats |
title_short | Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats |
title_sort | effect of scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of doca-salt hypertensive rats |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240798/ https://www.ncbi.nlm.nih.gov/pubmed/25422757 |
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